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Antibodies for G Proteins (Active and Inactive)

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Anti-Ras Mouse Monoclonal Antibody

Price$239.00
BrandNewEastBio   
Place of OriginUSA
Catalog Number26014
ImmunogenMouse
Size100 μl
Technical InformationView technical data
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  • Summary

    Ras [26014]
    [100 µL]


    Catalog Number:  26014
    Gene Symbol:  RAS
    Description:   Anti Ras Mouse Monoclonal Antibody
    Background:   Small GTPases are a super-family of cellular signaling regulators. Ras belongs to the Ras sub-family of GTPases that regulate cell proliferation, cell motility, and gene transcription. GTP binding increases the activity of Ras, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).
    Immunogen:   Recombinant full length protein of Ras
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:   ELISA 1:100-1:5000 
    WB 1:100-1:2000
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Ras antibody recognizes H-Ras, N-Ras and K-Ras from vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  image.png
    Western blot:  
    image.pngWestern blot analysis of Ras proteins. MEF whole-cell lysate was used for western blot with the monoclonal anti-Ras antibody (cat # 26014).
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    Publications:
    1.  DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model
        Gene Therapy 21, 888-896 (October 2014)
    2.  Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma
        J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136


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