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Antibodies for Mutated Oncogene

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Anti EGFR(T854A) Mouse Monoclonal Antibody

Price$495.00
BrandNewEastBio   
Place of OriginUSA
Catalog Number26481
ImmunogenMouse
Size100 μl
Technical InformationView technical data
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  • Summary

    EGFR(T854A)  26481
    [100ul]


    Catalog Number:  26481
    Gene Symbol:  EGFR/HER1/ERBB1
    Description:   Anti EGFR(T854A) Mouse Monoclonal Antibody
    Background:   EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).
    Immunogen:   A synthetic peptide from the internal region of EGFR which includes the mutation of T854A, human origin.
    Applications:   ELISA, WB, IF, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:5000 
    WB: 1:1000-1:5000 
    IHC: 1:50-1:200
    Concentration:   1 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes EGFR (T854A), but not wild type EGFR from vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  image.png
    Western blot:  
    image.pngWestern blot analysis of recombinant EGFR (T854A) and wildtype proteins. Purified His-tagged EGFR (T854A) protein (lane 1) and corresponding wild type protein (amino acids 702-987, lane 2) were blotted with anti EGFR(T854A) mouse monoclonal antibody (Cat. #26481).
    Immunofluorescence:  
    image.pngImmunofluorescence of cells expressing EGFR proteins with anti EGFR(T854A) antibody. HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP EGFR(T854A) plasmid (right column), then fixed and stained with anti EGFR(T854A) monoclonal antibody (Cat. #26481).


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