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Antibodies for Proto-oncogene


Anti BRaf Rabbit Polyclonal Antibody

Place of OriginUSA
Catalog Number21271
Size100 μl
Technical InformationView technical data
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  • Summary

    B-Raf [21271]
    [100 µL]

    Catalog Number:  21271
    Gene Symbol:  B-RAF, B-RAF1, RAFB1
    Description:   Anti BRaf Rabbit Polyclonal Antibody
    Background:   B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the B-RAF gene cause diseases. Inherited mutations in B-RAF cause cardiofaciocutaneous syndrome. Acquired mutations in B-RAF have been found in cancers.
    Immunogen:   Purified B-Raf protein, human origin.
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:5000 
    WB: 1:100-1:1000 
    IHC: 1:50-1:200
    Concentration:    0.1mg/ml
    Host:   Rabbit
    Format:   Liquid
    Clonality:   Polyclonal
    Purity:   Purified from serum
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes B-Raf of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with anti- B-Raf polyclonal antibody (Cat. #21271). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.

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    1.  Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma
        Mod Pathol. 2013 Mar;26(3):414-20.
    2.  Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients
        Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733
    3.  Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma
        human pathology. February 2015Volume 46, Issue 2, Pages 169–175
    4.  Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas
        human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570
    5.  BRAF V600E Mutation-Specific Antibody: A Review
        Seminars in Diagnostic Pathology

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