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Antibodies for GTP-bound G Proteins


Anti Arf6-GTP Mouse Monoclonal Antibody

Place of OriginUSA
Catalog Number26918
Size30 μl
Technical InformationView technical data
  • Data Sheet
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  • Summary

    Active Arf6-GTP [26918]
    [30 µL]

    Catalog Number:  26918
    Gene Symbol:  ARF6
    Description:   Anti-Active Arf6 Mouse Monoclonal Antibody
    Background:   ARF6 (ADP-ribosylation factor 6) is a member of the ARF super-family. ARF genes encode small GTPases that increase the ADP-ribosyltransferase activity of cholera toxin and are critical for vesicular trafficking as activators of phospholipase D. Arf6 regulates membrane trafficking and functions as a regulatory molecule of phagocytosis.
    Immunogen:   Recombinant full length protein of active Arf6.
    Applications:   IP, IHC
    Recommended Dilutions:   IP: 1 μg for 1~2 mg total cellular proteins 
    IHC: 1:50~100
    Concentration:    1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG
    Purity:    Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti-active Arf6 antibody recognizes active Arf6 from vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Immunoprecipitation/Western blot:  
    Purified full-length Arf6 proteins were immunoprecipitated after treated with GDP (lane left) or GTPγS (lane right). Immunoprecipitation was done with the anti-active Arf6 monoclonal antibody (Cat. No. 26918). Immunoblot was with an anti-Arf6 polyclonal antibody (Cat. No. 21032).
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