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Antibodies for GTP-bound G Proteins


Anti Ral-GTP Mouse Monoclonal Antibody

Place of OriginUSA
Catalog Number26913
Size30 μl
Technical InformationView technical data
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  • Summary

    Active Ral-GTP [26913]
    [30 µL]

    Catalog Number:  26913
    Gene Symbol:  Ral
    Description:   Anti Active Ral Mouse Monoclonal Antibody
    Background:   The Ras-like small G proteins, RalA and RalB, are important components of Ras signaling pathways, implicated in the initiation and maintenance of tumorigenic transformation, as well as vesicle transport, apoptosis, transcription, cell migration, and cell proliferation.
    Immunogen:   Recombinant full length protein of active RalB.
    Applications:   IP, IHC
    Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP; 
    1:100 dilution for IHC
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti-active Ral antibody recognizes active RalA and RalB of vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Immunoprecipitation/Western blot:  
    Ral activation assay. Purified RalB proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-active Ral monoclonal antibody (Cat. No. 26913). Immunoblot was with an anti-RalB rabbit polyclonal antibody (Cat. No. 21033).

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