NewEast Biosciences
A Helping Hand for Your Research
Phone:   610-945-2007      Fax:   877-271-1876
Advanced Search
                  
  
       
Documents
Publications Catalogue
Activation Assay Kits
Arf6 Arl1 Arl3
Cdc42 13 i
o s z
Rab11 Rab3 Rab35
Rab4A Rab5 Rab7
Rab5A Rab5B Rab5C
Rac1 Ran Rap1
RalA RalB Ras
RheB RhoA Sar1
Antibodies for GTP-bound G Proteins
Arf6 Arl1 Arl3
Cdc42 13 i
o s z
Rab11 Rab3 Rab35
Rab4A Rab5 Rab7
Ran Rac1 Ral
Rap1 Ras RheB
RhoA Sar1
cAMP/cGMP EIA Kits
without Acetylation
cAMP cGMP
Biomarkers
β-Actin β-Tubulin Albumin
Apo A1 BMP3 cAMP
cGMP CystatinC EYA2
Fascin GAPDH IDH1
Kim-1 MUC1 Orai1-L1
Orai1-L2 Ric-8A
Septin9 SLC7A5 SUMO-1
SUMO-2/3 TFF3
Vimentin Cystatin C EIA Kit
Transferrin Fascin EIA Kit
Polyclonal Antibodies
ApoA1 Arf1 Arf6
Bag1 Cdc42 Ch Cdc42
ERBB2 GRB7 12
13 i o
q s z
IDH1 IDH2 Orai1-L1
Orai1-L2 Sar1a Pan
Rab11 Rab12 Rab13
Rab14 Rab17 Rab18
Rab22 Rab25 Rab26
Rab28 Rab2A Rab30
Rab31 Rab35 Rab38
Rab39 Rab3C Rab3D
Rab41 Rab5 Rab5A
Rab5B Rab5C Rab6A
Rab9A Rab8A Rac1
Rac2 Ral RalA
RalB Ran RhoA
Ras RheB
Order
Fax     :  877-271-1876
Email  :  sale@neweastbio.com

Protocol for IF

  1. Rinse cells briefly in PBS (37°C, as quickly as possible).
  2. Aspirate PBS, cover cells with 300~500 µl 4% formaldehyde in PBS.
  3. Allow cells to fix for 15 minutes at room temperature.
  4. Aspirate fixative, rinse three times in PBS for 1 minute each.
  5. Block specimen in 5% FBS in PBS/Triton for 60 minutes.
  6. While blocking, prepare primary antibody by diluting 100 X in PBS/Triton (1:100 dilution).
    (You will need 200~300 µl per coverslip.)
  7. Aspirate blocking solution, apply diluted primary antibody.
    NOTE: For double-labeling, prepare a cocktail of mouse and rabbit primary antibodies at their appropriate dilutions in PBS/Triton.
  8. Incubate overnight at 4°C.
  9. Rinse 3X in PBS for 5 minutes each.
  10. Incubate with fluorochrome-conjugated secondary antibody diluted in PBS/Triton for 1-2 hours at room temperature in dark.
    NOTE: For double-labeling, prepare a cocktail of fluorochrome-conjugated anti-mouse and anti-rabbit primary antibodies at their appropriate dilutions in PBS/Triton.
  11. Rinse 3X in PBS for 5 minutes each.
  12. Examine specimens immediately using appropriate excitation wavelength, depending on fluorochrome for best results or store at 4°C in dark.
PBS/Triton: 1X PBS/0.3% Triton X-100
          Download Procedure
IHC/IF Publications
1.  Physiological Activation of Synaptic Rac> PAK (p-21 Activated Kinase) Signaling Is Defective in a Mouse Model of Fragile X Syndrome
    LY Chen, CS Rex, AH Babayan, EA Kramar, G Lynch, CM Gall and JC Lauterborn
     The Journal of Neuroscience, 2010
2.  Antioxidants Counteract Nicotine and Promote Migration via RacGTP in Oral Fibroblast Cells
    S Miguel, L Opperman, E Allen, J Zielinski and K Svoboda
     Journal of Periodontology, 2010
3.  K-Ras Mediated Murine Epidermal Tumorigenesis Is Dependent upon and Associated with Elevated Rac1 Activity
    MS Samuel, FC Lourenco and MF Olson
     OPLoS ONE, 2011 - dx.plos.org
4.  Resistance to inhibitors of cholinesterase-8A (Ric-8A) is critical for growth factor receptor-induced actin cytoskeletal reorganization
     J. Biol. Chem. 2011
5.  Enterohemorrhagic Escherichia coli infection stimulates Shiga toxin 1 macropinocytosis and transcytosis across intestinal epithelial cells
     American Journal of Physiology - Cell Physiology. 2011
View IHC/IF Images
Active Gα13-GTP (26902) on MEF cells
Active Cdc42-GTP (26905) on MS1 cells
Active Rac1-GTP (26903) on brain tissue sections
Protocols
Protocol for IF FAQs
Antibodies for Mutated Oncogenes
Ret Abl
AKT1 ALK
ATM B-Raf
BRCA1 BRCA2
CTNNB1 CTCF
EGFR ERBB2
EML4-ALK FGFR1
GNAQ IDH1
IDH2 JAK2
MEK1 NFE2L2
NOTCH PIK3CA
RHEB Ras
TP53 SMAD4
STK11 SMO
KIT
For WB Only (FREE SAMPLE)
ALK (D1225N) BRAF (D594G)
ALK (R1275Q) EGFR (L858Q)
MEK1 (E203K) KIT (V560D)
KIT (K642E) KIT (H697Y)
PIK3CA (G1049S) PIK3CA (E542K)
PIK3CA (Q546R) PIK3CA (Q546P)
EGFR (E746-A750)del Β-catenin (A43V)
Monoclonal Antibodies
Arf1 Arf6 Arl1
Arl3 Bag1 Cdc42
FGFR1 GNAQ 12
13 14 i
o q s
z Rab3A Rab35
Rab5 Rab7 Rab11
Rac1 Rac3 Ran
Ral RalB Ras
RheB RhoA Sar1
NFE2L2 His-tag Vimentin
Purified Proteins
Arf1 Δ17 Arf6
Arf6 Δ12 Q67L Arl1 Q71L
Cdc42 G12V Cdc42 His6-tag
CST3 Fascin1
Gα13 Gαi1
Gαi2 Q205L Rab3A Q81L
Rab35 Q67L Rab4A Q67L
Rab5 Rab5 Q79L
Rab7 Rab7 Q67L
Rac RalB
Ran Ran Q69L
Ras Ras Q61L
RhoA Sar1a
EGFR protein, L858R
NFE2L2 Protein D29G mutant
NFE2L2 Protein D77V mutant
NFE2L2 Protein E79K mutant
NFE2L2 Protein E79Q mutant
NFE2L2 Protein L30F mutant
NFE2L2 Protein R34Q mutant
NFE2L2 Protein V32T mutant
more...