|Place of Origin||USA|
|Technical Information||View technical data|
Gαi2 Protein Q205L mutant 
|Catalog Number: 10147|
|Product Name: Gαi2 Protein Q205L mutant|
|Synonyms: Guanine nucleotide binding protein, alpha inhibiting activity polypeptide 2, GNAI2, Galphai2|
|Source: Human, recombinant full length, His6-tag|
|Expression Host: E. coli|
|Molecular Weight: 40 kDa|
|Purity: >95% by SDS-PAGE|
|Introduction: Heterotrimeric G proteins are critical cellular signal transducers. Gαi represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαi proteins are being explored.|
|Amino Acid Sequence (1-355, Q205L)|
LRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDL FEEKITHSPLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVI IKNNLKDCGLF
|Physical Appearance (form): Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl|
|Physical Appearance (form): White or clear|
|Concentration: 1 mg/mL|
|Preparation Instructions: |
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Gαi2 Q205L was determined by SDS-PAGE and Coomassie Brilliant Blue Staining.
1. Blatt, C. et al., Proc. Nat. Acad. Sci. 85: 7642-7646, 1988.
2. Bloch, D. B. et al., Am. J. Hum. Genet. 42: 884-888, 1988.
3. Bray, P. et al., Proc. Nat. Acad. Sci. 84: 5115-5119, 1987.
4. Itoh, H. et al., J. Biol. Chem. 263: 6656-6664, 1988.
5. Lan, K.-L. et al., J. Biol. Chem. 273: 12794-12797, 1998.
6. Neer, E. J. et al., Hum. Genet. 77: 259-262, 1987.
7. Ogden, S. K. et al., Nature 456: 967-970, 2008.
|1. Identification of novel signalling roles and targets for Galpha and Gbetagamma downstream of the insulin-like growth factor 1 receptor in vascular smooth muscle cells|
Biochem J. 2013 Feb 15;450(1):209-19
|2. CRH activation of different signaling pathways results in differential calcium signaling in human pregnant myometrium before and during labor|
J Clin Endocrinol Metab. 2012 Oct;97(10):E1851-61
|3. CXCR4 activation defines a new subgroup of Sonic hedgehog-driven medulloblastoma|
Cancer Res. 2012 Jan 1;72(1):122-32
|4. Regulation of estradiol and progesterone production by CRH-R1 and -R2 is through divergent signaling pathways in cultured human placental trophoblasts|
Endocrinology. 2012 Oct;153(10):4918-28
|5. WNT-5A stimulates the GDP/GTP exchange at pertussis toxin-sensitive heterotrimeric G proteins|
Cell Signal. 2011 Mar;23(3):550-4
|6. Heterotrimeric Galpha(i) proteins are regulated by lipopolysaccharide and are anti-inflammatory in endotoxemia and polymicrobial sepsis|
Biochim Biophys Acta. 2011 Mar;1813(3):466-72
|7. Estrogen- and xenoestrogen-induced ERK signaling in pituitary tumor cells involves estrogen receptor-α interactions with G protein-αi and caveolin I|
Steroids Volume 77, Issue 5, April 2012, Pages 424–432
|8. Na/H exchanger regulatory factors control parathyroid hormone receptor signaling by facilitating differential activation of G(alpha) protein subunits|
J Biol Chem. 2010 Aug 27;285(35):26976-86
|9. Beta-arrestin- but not G protein-mediated signaling by the "decoy" receptor CXCR7|
Proc Natl Acad Sci U S A. 2010 Jan 12;107(2):628-32
|10. Structural basis for activation of trimeric Gi proteins by multiple growth factor receptors via GIV/Girdin|
Mol Biol Cell. 2014 Nov 5;25(22):3654-71
|11. Canonical and Non-canonical G-protein Signaling Helps Coordinate Actin Dynamics to Promote Macrophage Phagocytosis of Zymosan|
Mol Cell Biol. 2014 Nov 15;34(22):4186-99
|12. Fatty Acid-binding Protein 5 (FABP5) Regulates Cognitive Function Both by Decreasing Anandamide Levels and by Activating the Nuclear Receptor Peroxisome Proliferator-activated Receptor β/δ (PPARβ/δ) in the Brain|
J Biol Chem. 2014 May 2;289(18):12748-58