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G Proteins and Oncogene

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Fascin1 Protein

Price$239.00
BrandNewEastBio   
Place of OriginUSA
Catalog Number10150
ImmunogenE. coli
Size100 μl
Technical InformationView technical data
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  • Summary

    Fascin1 Protein [10150]
    [100 µg]


    Catalog Number:  10150
    Product Name:  Fascin1 Protein
    Synonyms:  Fascin homolog 1, actin-bundling protein, FSCN1, SNL, p55
    Source:   Human, recombinant full length, his-tag
    Expression Host:   E. coli
    Molecular Weight:   55 kDa
    Purity:   >95% by SDS-PAGE
    Introduction:   Fascin is an ~58 kDa monomeric actin filament bundling protein. It is required to maximally cross-link the actin filaments into straight, compact, and rigid bundles, and contributes to the formation of filopodia that are critical for cell migration. Elevated levels of fascin have been found in metastatic tumors and are correlated with clinically aggressive phenotypes, poor prognosis, and shorter survival.
    Amino Acid Sequence   (1-493)
    MTANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPDEAGSAAVCLRSHLG 
    RYLAADKDGNVTCEREVPGPDCRFLIVAHDDGRWSLQSEAHRRYFGGTEDRLSCFAQTVSPAEKWSV 
    HIAMHPQVNIYSVTRKRYAHLSARPADEIAVDRDVPWGVDSLITLAFQDQRYSVQTADHRFLRHDGRLVA 
    RPEPATGYTLEFRSGKVAFRDCEGRYLAPSGPSGTLKAGKATKVGKDELFALEQSCAQVVLQAANERNV 
    STRQGMDLSANQDEETDQETFQLEIDRDTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRD 
    RRITLRASNGKFVTSKKNGQLAASVETAGDSELFLMKLINRPIIVFRGEHGFIGCRKVTGTLDANRSSYD 
    VFQLEFNDGAYNIKDSTGKYWTVGSDSAVTSSGDTPVDFFFEFCDYNKVAIKVGGRYLKGDHAGVLKA 
    SAETVDPASLWEY
    Properties
    Physical Appearance (form):   Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.
    Physical Appearance (form):  White or clear.
    Concentration:  1 mg/mL
    Storage:   -80°C
    Preparation Instructions:  
    Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside(DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His6-tagged. Fascin was determined by SDS-PAGE and Coomassie Brilliant Blue Staining
    References:  
    1. Mosialos, G. et al., Am. J. Path. 148: 593-600, 1996. 
    2. Ono, S. et al., J. Biol. Chem. 272: 2527-2533, 1997. 
    3. Pinkus, G. S. et al., Am. J. Path. 150: 543-562, 1997.
    Datasheet:  


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