|Place of Origin||USA|
|Technical Information||View technical data|
RhoA Protein 
|Catalog Number: 10104|
|Product Name: RhoA Protein|
|Synonyms: Ras homolog gene family, member A, ARHA, ARH12, RHO12, RHOH12|
|Source: Human, recombinant full length, His6-tag|
|Expression Host: E. coli|
|Molecular Weight: 22 kDa|
|Purity: >96% by SDS-PAGE|
|Introduction: Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca2+ sensitivity of smooth muscle contraction.|
|Amino Acid Sequence (1-193)|
|Physical Appearance (form): Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.|
|Physical Appearance (form): White or clear|
|Concentration: 1 mg/mL|
|Preparation Instructions: |
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged RhoA was determined by SDS- PAGE and Coomassie Brilliant Blue Staining.
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|1. Ethanol increases p190RhoGAP activity, leading to actin cytoskeleton rearrangements|
J Neurochem. 2011 Dec;119(6):1306-16
|2. The transcriptional coactivators megakaryoblastic leukemia 1/2 mediate the effects of loss of the tumor suppressor deleted in liver cancer 1|
Oncogene. 2012 Aug 30;31(35):3913-23
|3. G(alpha)12/13 inhibition enhances the anticancer effect of bortezomib through PSMB5 downregulation|
Carcinogenesis. 2010 Jul;31(7):1230-7
|4. Transgenesis of the Wolffian duct visualizes dynamic behavior of cells undergoing tubulogenesis in vivo|
Development, Growth & Differentiation. Volume 55, Issue 4, pages 579–590, May 2013
|5. Foxc2 induces Wnt4 and Bmp4 expression during muscle regeneration and osteogenesis|
Cell Death and Differentiation (2013) 20, 1031–1042
|6. N-glycosylation induces collagen triple helix repeat containing 1 (CTHRC1) and drives oral cancer cell migration|
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|7. Cyclodextrin protects podocytes in diabetic kidney disease|
Diabetes. 2013 Nov;62(11):3817-27
|8. Amyloid beta1-42 (Aβ42) up-regulates the expression of sortilin via the p75NTR/RhoA signaling pathway|
Journal of Neurochemistry Volume 127, Issue 2, pages 152–162, October 2013
|9. Podocyte-specific GLUT4 deficient mice have fewer and larger podocytes and are protected from diabetic nephropathy|
Diabetes. 2014 Feb;63(2):701-14
|10. Loss of TAK1 increases cell traction force in a ROS-dependent manner to drive epithelial–mesenchymal transition of cancer cells|
Cell Death and Disease (2013) 4, e848
|11. Syndecan-2 regulation of morphology in breast carcinoma cells is dependent on RhoGTPases|
Biochimica et Biophysica Acta (BBA) - General Subjects Volume 1840, Issue 8, August 2014, Pages 2482–2490
|12. Epithelial repair is a two-stage process driven first by dying cells and then by their neighbours|
J Cell Sci. 2014 Mar 15;127(Pt 6):1229-41
|13. MIF inhibits monocytic movement through a non-canonical receptor and disruption of temporal Rho GTPase activities in U-937 cells|
Cytokine Volume 69, Issue 1, September 2014, Pages 47–55
|14. Triclosan Potentiates Epithelial-To-Mesenchymal Transition in Anoikis-Resistant Human Lung Cancer Cells|
PLoS One. 2014 Oct 16;9(10):e110851