|Place of Origin||USA|
|Technical Information||View technical data|
RhoA Protein 
|Catalog Number: 10104|
|Product Name: RhoA Protein|
|Synonyms: Ras homolog gene family, member A, ARHA, ARH12, RHO12, RHOH12|
|Source: Human, recombinant full length, His6-tag|
|Expression Host: E. coli|
|Molecular Weight: 22 kDa|
|Purity: >96% by SDS-PAGE|
|Introduction: Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca2+ sensitivity of smooth muscle contraction.|
|Amino Acid Sequence (1-193)|
|Physical Appearance (form): Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.|
|Physical Appearance (form): White or clear|
|Concentration: 1 mg/mL|
|Preparation Instructions: |
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged RhoA was determined by SDS- PAGE and Coomassie Brilliant Blue Staining.
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9. Yoshida, S.et al., Science 313: 108-111, 2006.
|80601||RhoA Activation Assay Kit||20 assays||$737|
|26904||Active RhoA-GTP Monoclonal Antibody||30 μL||$495|
|26007||Anti-RhoA Mouse Monoclonal Antibody||100 μL||$239|
|21017||Anti-RhoA Rabbit Polyclonal Antibody||100 μL||$219|
|10104||RhoA Protein||100 μL|| $239 |
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|10. Loss of TAK1 increases cell traction force in a ROS-dependent manner to drive epithelial–mesenchymal transition of cancer cells|
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|11. Syndecan-2 regulation of morphology in breast carcinoma cells is dependent on RhoGTPases|
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