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ILK Rabbit pAb

Anti-ILK Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216582

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216582
Product Name:   Anti-ILK Rabbit Polyclonal Antibody
Synonyms:   P59; ILK-1; ILK-2; p59ILK
UNIPROT ID:   Q13418 (Gene Accession – BC001554 )
Background:    This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants.
Immunogen:    Fusion protein of human ILK
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 500-2000;ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 216582(ILK Antibody) at a dilution of 1/60(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 216582(Anti-ILK Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 216582(Anti-ILK Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with fusion protein and then with D220857(Anti-ILK Antibody) at dilution 1/60.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Mouse heart tissue, hela cells;
Primary antibody: 216582(ILK Antibody) at dilution 1/600;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 40 seconds
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