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ACTA1 Rabbit pAb

Anti-ACTA1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216988

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216988
Product Name:   Anti-ACTA1 Rabbit Polyclonal Antibody
Synonyms:   ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM
UNIPROT ID:   P68133 (Gene Accession – BC012597 )
Background:    The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
Immunogen:    Fusion protein of human ACTA1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 216988(ACTA1 Antibody) at a dilution of 1/65(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 216988(Anti-ACTA1 Antibody) at dilution 1/65.

The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 216988(Anti-ACTA1 Antibody) at a dilution of 1/65.

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with fusion protein and then with D221592(Anti-ACTA1 Antibody) at dilution 1/65.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Mouse muscle tissue, Mouse heart tissue;
Primary antibody: 216988(ACTA1 Antibody) at dilution 1/500;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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