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GOT1 Rabbit pAb

Anti-GOT1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217190

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217190
Product Name:   Anti-GOT1 Rabbit Polyclonal Antibody
Synonyms:   cCAT; GIG18; cAspAT; ASTQTL1
UNIPROT ID:   P17174 (Gene Accession – BC000498 )
Background:    Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
Immunogen:    Fusion protein of human GOT1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 217190(GOT1 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 217190(Anti-GOT1 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 217190(Anti-GOT1 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with fusion protein and then with D221954(Anti-GOT1 Antibody) at dilution 1/50.

Gel: 15%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Human normal liver tissue, mouse brain tissue, Mouse heart tissue;
Primary antibody: 217190(GOT1 Antibody) at dilution 1/1600;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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