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NDUFS3 Rabbit pAb

Anti-NDUFS3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217637

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217637
Product Name:   Anti-NDUFS3 Rabbit Polyclonal Antibody
Synonyms:   CI-30
UNIPROT ID:   O75489 (Gene Accession – BC000617 )
Background:    This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.
Immunogen:    Fusion protein of human NDUFS3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 217637(NDUFS3 Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 217637(Anti-NDUFS3 Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 217637(Anti-NDUFS3 Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with fusion protein and then with D222746(Anti-NDUFS3 Antibody) at dilution 1/40.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-6: 293T cells, NIH/3T3 cells, Raw264.7 cells, hela cells, human fetal brain tissue, mouse brain tissue;
Primary antibody: 217637(NDUFS3 Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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