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NDUFS4 Rabbit pAb

Anti-NDUFS4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217640

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217640
Product Name:   Anti-NDUFS4 Rabbit Polyclonal Antibody
Synonyms:   AQDQ; CI-18
UNIPROT ID:   O43181 (Gene Accession – BC005270 )
Background:    This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), or NADH:ubiquinone oxidoreductase, the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone. In well-coupled mitochondria, the electron flux leads to ATP generation via the building of a proton gradient across the inner membrane.
Immunogen:    Fusion protein of human NDUFS4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 217640(NDUFS4 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the fusion protein and then with 217640(Anti-NDUFS4 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 217640(Anti-NDUFS4 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with fusion protein and then with D222749(Anti-NDUFS4 Antibody) at dilution 1/20.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Mouse kidney tissue, Mouse heart tissue;
Primary antibody: 217640(NDUFS4 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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