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PAM16 Rabbit pAb

Anti-PAM16 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219194

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219194
Product Name:   Anti-PAM16 Rabbit Polyclonal Antibody
Synonyms:   TIM16; MAGMAS; SMDMDM; TIMM16; CGI-136
UNIPROT ID:   Q9Y3D7 (Gene Accession – BC005024 )
Background:    This gene encodes a mitochondrial protein involved in granulocyte-macrophage colony-stimulating factor (GM-CSF) signaling. This protein also plays a role in the import of nuclear-encoded mitochondrial proteins into the mitochondrial matrix and may be important in reactive oxygen species (ROS) homeostasis. Mutations in this gene cause Megarbane-Dagher-Melike type spondylometaphyseal dysplasia, an early lethal skeletal dysplasia characterized by short stature, developmental delay and other skeletal abnormalities.
Immunogen:    Fusion protein of human PAM16
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 219194(PAM16 Antibody) at a dilution of 1/60(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 219194(Anti-PAM16 Antibody) at dilution 1/60.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: HL-60, Hela, Jurkat, LNCAP, HepG2 cell lysates;
Primary antibody: 219194(PAM16 Antibody) at dilution 1/900;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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