Email: info@neweastbio.com   |  Call: 6109452007

LAT Rabbit pAb

Anti-LAT Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221459

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221459
Product Name:   Anti-LAT Rabbit Polyclonal Antibody
Synonyms:   LAT1; pp36
UNIPROT ID:   O43561 (Gene Accession – NP_055202 )
Background:    The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms.
Immunogen:    Synthetic peptide of human LAT
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 221459(LAT Antibody) at a dilution of 1/30(Cell membrane and Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 221459(Anti-LAT Antibody) at dilution 1/30.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: Human lymph gland tissue lysate;
Primary antibody: 221459(LAT Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
          Publications