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p53 (2D10) Mouse mAb

Anti-p53 (2D10) Mouse Monoclonal Antibody
5/5

$349.00

Cat.#:  N261271

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N261271
Product Name:   Anti-p53 (2D10) Mouse Monoclonal Antibody
Synonyms:   TP53; P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
UNIPROT ID:   P04637
Background:    Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains.
Immunogen:    Synthetic Peptide of p53
Applications:    WB,IHC-F,IHC-P,ICC/IF
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    2D10-1E9-1D3
MW:    Calculated MW: 44 kDa; Observed MW: 53 kDa
Isotype:    IgG1
Purification:    Affinity Purified
Species Reactivity:    Human
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human colon cancer tissue using p53 (2D10) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunofluorescence analysis of p53 in Hela using p53 (2D10) antibody (Left) and DAPI (Right).

Western blot analysis of p53 (2D10) in T47D and COS7 lysates using p53 (2D10) antibody
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