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Phospho-BRAF (Thr401) Rabbit pAb

Anti-Phospho-BRAF (Thr401) Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  N225743

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N225743
Product Name:   Anti-Phospho-BRAF (Thr401) Rabbit pAb
Synonyms:   BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1
UNIPROT ID:   P15056
Background:    Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations.
Immunogen:    A synthesized peptide derived from human Phospho-B Raf (T401)
Applications:    WB,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Clone ID:   
MW:    Calculated MW: 84 kDa; Observed MW: 84 kDa
Isotype:    IgG
Purification:    Affinity Chromatography
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Phosphorylated
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Western blot analysis of Phospho-B Raf (T401) in (1) PC-12 lysates; (2) PC-12 lysates treated with TPA using Phospho-BRAF (Thr401) antibody.
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