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BCL2 Rabbit pAb

Anti-BCL2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219669

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219669
Product Name:   Anti-BCL2 Rabbit Polyclonal Antibody
Synonyms:   Bcl-2; PPP1R50
UNIPROT ID:   P10415 (Gene Accession – NP_000624 )
Background:    This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
Immunogen:    Synthetic peptide of human BCL2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 30-150;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer, Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 219669(BCL2 Antibody) at a dilution of 1/20(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the synthetic peptide and then with 219669(Anti-BCL2 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 219669(Anti-BCL2 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with synthetic peptide and then with D260117(Anti-BCL2 Antibody) at dilution 1/20.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: Jurkat, Raji, 293T, MCF-7 cell lysates;
Primary antibody: 219669(BCL2 Antibody) at dilution 1/1300;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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