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NDUFS1 Rabbit pAb

Anti-NDUFS1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217638

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217638
Product Name:   Anti-NDUFS1 Rabbit Polyclonal Antibody
Synonyms:   CI-75k; CI-75Kd; PRO1304
UNIPROT ID:   P28331 (Gene Accession – BC012068 )
Background:    The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme.
Immunogen:    Fusion protein of human NDUFS1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer, Metabolism, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 217638(NDUFS1 Antibody) at a dilution of 1/35(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the fusion protein and then with 217638(Anti-NDUFS1 Antibody) at dilution 1/35.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 217638(Anti-NDUFS1 Antibody) at a dilution of 1/35.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D222747(Anti-NDUFS1 Antibody) at dilution 1/35.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: 293T cells, PC3 cells, hela cells, Jurkat cells, Raji cells;
Primary antibody: 217638(NDUFS1 Antibody) at dilution 1/650;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 seconds
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