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NFATC1 Rabbit pAb

Anti-NFATC1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216675

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216675
Product Name:   Anti-NFATC1 Rabbit Polyclonal Antibody
Synonyms:   NFAT2; NFATc; NF-ATC; NF-ATc1.2
UNIPROT ID:   O95644 (Gene Accession – BC112243 )
Background:    The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes.
Immunogen:    Fusion protein of human NFATC1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Epigenetics and Nuclear Signaling, Cardiovascular, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 216675(NFATC1 Antibody) at a dilution of 1/75(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 216675(Anti-NFATC1 Antibody) at dilution 1/75.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: RAW264.7, 293T, Ramos cell lysates;
Primary antibody: 216675(NFATC1 Antibody) at dilution 1/1300;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 5 seconds
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