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Smad3 (4H4) Mouse mAb

Anti-Smad3 (4H4) Mouse Monoclonal Antibody
5/5

$349.00

Cat.#:  N261377

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N261377
Product Name:   Anti-Smad3 (4H4) Mouse Monoclonal Antibody
Synonyms:   SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
UNIPROT ID:   P84022
Background:    Smad3 transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton.
Immunogen:    Purified recombinant protein expressed in E.coli.
Applications:    WB,IHC-P
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    4H4-10A10-7B4
MW:    Calculated MW: 48 kDa; Observed MW: 48 kDa
Isotype:    IgG1
Purification:    Affinity Purified
Species Reactivity:    Human,Rat,Mouse
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human Liver Tissue using Smad3 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of Smad3 (4H4)in 293T, Jurkat, rat Heart, mouse Heart lysates using Smad3 antibody.

Immunohistochemistry analysis of paraffin-embedded Human Colon Carcinoma using Smad3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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