Mouse Monoclonal Antibody Service
Monoclonal antibodies (mAbs) have a much higher specificity and lower between-batch variability compared to pAbs.
Monoclonal antibodies are a class of antibodies that are produced by hybridoma cells by cell fusion technology and can bind to specific antigenic epitopes. Monoclonal antibodies have the characteristics of batch stability, high specificity and easy large-scale preparation, so they are widely used in biological research, in vitro diagnosis and drug development. According to the different needs of customers, we customize different solutions according to the type, quantity and purity of their antigens; through HAT screening and multiple subcloning screening to ensure 100% purity of cell lines; we use antibody affinity purification to ensure high antibody purity, Whether you use it downstream for diagnostic testing or drug research, its effectiveness is guaranteed.
| Step | Details | Timeline |
|---|---|---|
| Antigen design and preparation | Recombinant protein production and peptide synthesis and conjugation | 1-3 Weeks |
| First Immunization | Subcutaneous multipoint immunization | 4 Week |
| First Booster | Subcutaneous multipoint immunization | 7 Weeks |
| Second Booster | Subcutaneous multipoint immunization | 9 Weeks |
| Blood Test | 50 µL of serum was collected from each mouse, and the titer was detected by ELISA | 10 Week |
| Third Booster | Subcutaneous multipoint immunization | 11 Week |
| Shock Immunity | Intraperitoneal or intravenous immunization | 12 Week |
| Cell Fusion | Select the mice with the best immune effect for cell fusion | 13 Week |
| Cell Screening | Screening of positive cell lines by ELISA and other experimental methods | 14 Week |
| Ascites preparation and purification | For WB antibody
1、 5 BALB/c mice will be immunized, and the best mouse will be chosen for fusion. 2、 1-3 positive clones will be delivered. 3、 1 clone will be used to make ascites followed by purification. For application-specific antibody (IHC, IF, IP, ChIP) 1、 10 mice will be immunized, and the best 2 mice will be chosen for fusion. 2、 1-3 positive clones will be delivered. 3、 3-5 clones will be used to make ascites followed by purification. | 17 Week |
| Quality Appraisal | According to the customer’s downstream application, carry out corresponding quality inspection | 21 Week |
| Subtyping | Subtyping of all positive cell lines and purified antibodies | 22 Week |
Remark
- Host: BLAB/C mice;
- Antigen requirements: concentration>1mg/ml, purity>85%, and the antigen solution does not contain organic solvents, tris, amino acids, etc.
Deliverables
- 3-5 strains of hybridoma cells
- > 5 mg purified antibody
- 100 µL ascites stock solution
- Project report, including a complete set of data on antigen production, hybridoma construction, screening, cloning and related testing
Custom Polyclonal Antibody Production
We has more than ten years of experience in antibody preparation and is well versed in the key aspects of polyclonal antibody preparation. The host for the antibody can be rabbits, mice, rats, guinea pigs, hamsters, goats, chickens or other species. The titer of polyclonal antibodies can reach more than 1:10000. Whether the antigen is recombinant proteins, peptides and virus particles, we can provide you with a complete set of solutions from target design, antigen preparation to production, purification and quality testing, ensuring that the prepared antibodies can be effectively used in project research.
Highlights
Free Antigen Design – Based on experience, data, and characteristics of your desired target.
- Wider Choice of Host Animals – Choose from SPF (specific-pathogen-free) host animals, including rabbit, mouse, rat, and guinea pig.
- High Specificity – Antibodies are purified by Protein A and antigen affinity chromatography. When needed, we can provide cross-purification to obtain a more specific antibody.
- Guaranteed Result – 1 ng of protein antigen or 100 ng of peptide antigen is detected when the antibody is diluted at 1:6000.
Process Workflow
| Step | Details | Timeline |
|---|---|---|
| Antigen design and preparation | Recombinant protein production and peptide synthesis and conjugation | Week 1-3 |
| Negative serum collection | Collect 3-5 ml control serum before immunization | Week 2-3 |
| First Immunization | Subcutaneous multipoint immunization | Week 2-3 |
| First Booster | Subcutaneous multipoint | Week 4-5 |
| Second Booster | Subcutaneous multipoint | Week 6-7 |
| Blood Test | 50 µL of serum was collected from each mouse, and the titer was detected by ELISA | Weeks 7-8 |
| Third Booster | Subcutaneous multipoint immunization | Weeks 9-10 |
| Final blood collection | 20 ml serum was collected from each rabbit, and the titer was detected by ELISA | 12 Week |
| Shock Immunity | Ear vein immunity | Weeks 11-12 |
| Final blood collection | Collect 40-50 ml of blood from each rabbit | Week 12-13 |
| Antiserum Purification | Choose protein A or antigen affinity purification according to customer requirements | Week 14-15 |
| Project Delivery | Antibody delivery and project production report | Weeks 15-16 |
Remark
- Host: New Zealand White Rabbit
- Antigen requirements: concentration>1mg/ml, purity>85%, and the antigen solution does not contain organic solvents, tris, amino acids, etc.
Deliverables
- 200 μL pre-immune negative serum (50 μL for mice);
- More than 1 mg of antigens or polypeptides prepared by Primetal;
- 3-5 mg of antigen affinity-purified polyclonal antibody (concentration > 1 mg/mL);