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Rheb-GTP

Anti-Rheb-GTP Mouse Monoclonal Antibody
5/5

$495.00

Cat.#:  26910

   Size:   30 μl

In Stock

            Product Description          
Rheb-GTP
Cat. #:   26910
Size:  30 µL
Gene Symbol:  RHEB
Description:   Anti-Rheb-GTP Monoclonal Antibody
Background:   Small GTPases are a super-family of cellular signaling regulators. Rheb is a member of the Ras-superfamily GTPases. Rheb has been shown to interact with C-Raf, Mammalian target of rapamycin (mTOR), TSC2, Ataxia telangiectasia mutated (ATM), KIAA1303 and Ataxia telangiectasia and Rad3 related (ATR).
Immunogen:   Recombinant full-length protein of active Rheb.
Applications:   IP, IHC, IF
Recommended Dilutions:    IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgM
Purity:   Purified from ascites
Preservative:  No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-Rheb-GTP monoclonal antibody recognizes active Rheb of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze/thaw cycles
Immunoprecipitation/Western blot:   Rheb activation assay. Purified Rheb proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-Rheb-GTP monoclonal antibody (Cat. No. 26910). Immunoblot was with an anti-Rheb rabbit polyclonal antibody (Cat. No. 21098).
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01. Mnk1/2 kinases regulate memory and autism-related behaviours via Syngap1
Brain. 2023   PMID: 36315645
02. Mnk1/2 kinases regulate memory and autism-related behaviours via Syngap1
Brain. 2022  PMID: 36315645
03. NADPH Oxidase 2 Derived Reactive Oxygen Species Promote CD8+ T cell Effector Function
bioRxiv. 2021
04. PIR-B Regulates CD4+ IL17a+ T-Cell Survival and Restricts T-Cell-Dependent Intestinal Inflammatory Responses
Cell Mol Gastroenterol Hepatol. 2021  PMID: 34242819
05. Cardiac-specific deletion of FDPS induces cardiac remodeling and dysfunction by enhancing the activity of small GTP-binding proteins
J Pathol. 2021  PMID: 34467534
06. RNA N6-Methyladenosine Methyltransferase METTL3 Facilitates Colorectal Cancer by Activating the m6A-GLUT1-mTORC1 Axis and Is a Therapeutic Target.
Gastroenterology. 2020  PMID: 33217448
07. CBAP modulates Akt-dependent TSC2 phosphorylation to promote Rheb-mTORC1 signaling and growth of T-cell acute lymphoblastic leukemia
Oncogene. 2019  PMID: 30266989
08. Oncogenic KRAS signaling activates mTORC1 through COUP-TFII-mediated lactate production
EMBO Rep. 2019  PMID:  30988000
09. Ubiquitination of Rheb governs growth factor-induced mTORC1 activation
Cell Res. 2018  PMID:  30514904
10. Mammalian target of rapamycin complex 1 activation sensitizes human glioma cells to hypoxia-induced cell death
Brain. 2017  PMID: 28969371
11. Alteration of protein prenylation promotes spermatogonial differentiation and exhausts spermatogonial stem cells in newborn mice
Sci Rep. 2016  PMID: 27374985
12. Normalizing translation through 4E-BP prevents mTOR-driven cortical mislamination and ameliorates aberrant neuron integration
Proc Natl Acad Sci U S A. 2016  PMID: 27647922
13. Suppression of the GTPase-activating protein RGS10 increases Rheb-GTP and mTOR signaling in ovarian cancer cells
Cancer Lett. 2015  PMID:  26319900
14. Altered nutrient response of mTORC1 as a result of changes in REDD1 expression: effect of obesity vs. REDD1 deficiency
J Appl Physiol. 2014   PMID: 24876363
15. Inducible Nitric Oxide Synthase Drives mTOR Pathway Activation and Proliferation of Human Melanoma by Reversible Nitrosylation of TSC2
Cancer Res. 2014  PMID: 24398473
16. REDD1 enhances protein phosphatase 2A-mediated dephosphorylation of Akt to repress mTORC1 signaling
Sci Signal. 2014  PMID: 25056877
17. SynGAP Regulates Protein Synthesis and Homeostatic Synaptic Plasticity in Developing Cortical Networks
PLoS One. 2013  PMID: 24391850
18. Rheb/mTORC1 Signaling Promotes Kidney Fibroblast Activation and Fibrosis
J Am Soc Nephrol. 2013  PMID: 23661807