Email:   |  Call: 6109452007


Anti-Ras(G13D) Mouse Monoclonal Antibody


Cat.#:  26038

   Size:   100 μl

In Stock

          Product Description          
Cat. #:  26038
Gene Symbol:  Ras(G13D)
Description:   Anti-Ras(G13D) Mouse Monoclonal Antibody
Background:   Ras(G13D) is one type of Ras missense mutations at the codon 13. Ras protein is a membrane-associated GTPase that regulates cell proliferation, differentiation, and survival. Ras G13D mutation results in decreased GTPase activity and constitutive signaling. This mutation is found in many types of human cancers.
Immunogen:    A synthetic peptide from the internal region of Ras which includes the mutation of G13D, human origin.
Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:    ELISA: 1:100-1:3000 WB: 1:200-1:1000 IF: 1:50-1:100 IHC: 1:50-1:100
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG2b
Purity:   Purified from ascites
Preservative: No
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl and 50% glycerol
Species Reactivity:  Anti-Ras(G13D) monoclonal antibody recognizes RasG13D proteins of vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing
Western blot:   Western blot analysis of recombinant Ras WT and G13D proteins with anti-Ras(G13D) monoclonal antibody. Equal amounts of purified Ras WT (lane 1, Cat # 10172) and G13D (lane 2, Cat # 10155) proteins were loaded and blotted with anti-Ras(G13D) mouse monoclonal antibody (Cat # 26038) (top panel). Input control (bottom panel) was blotted with rabbit polyclonal anti-Ras antibody (Cat # 21021). Immunofluorescence:   Immunofluorescence of cells expressing RAS proteins with Anti-Ras(G13D) antibody. HEK293T cells were transfected with pCDNA3-GFP-Ras(G13D) plasmid,pCDNA3-GFP-Ras(WT)plasmid, pCDNA3-GFP-Ras(G13A) plasmid,pCDNA3-GFP-Ras(G13V) plasmid, pCDNA3-GFP-Ras(G13C)plasmid, pCDNA3-GFP-Ras(G12R)plasmid or pCDNA3-GFP-Ras(G13S)plasmid, then fixed and stained with anti-Ras(G13D) monoclonal antibody (Cat. # 26038).
          Related Products          
01. SF3B1 facilitates HIF1-signaling and promotes malignancy in pancreatic cancer
Cell Rep. 2022
02. Membrane Anchorage-Induced (MAGIC) Knockdown of Non-synonymous Point Mutations
Chembiochem. 2022
03. The GSK3 kinase and LZTR1 protein regulate the stability of Ras family proteins and the proliferation of pancreatic cancer cells
Neoplasia. 2022
04. SF3B1 promotes tumor malignancy through splicing-independent activation of HIF1à
bioRxiv, 2020
05. Selective targeting of the oncogenic KRAS G12S mutant allele by CRISPR/Cas9 induces efficient tumor regression
Theranostics. 2020
06. Transcriptional and metabolic rewiring of colorectal cancer cells expressing the oncogenic KRASG13D mutation
Br J Cancer. 2019
07. A novel pancreatic cancer model originated from transformation of acinar cells in adult tree shrew, a primate-like animal
Dis Model Mech. 2019
08. Roles of CD133 in microvesicle formation and oncoprotein trafficking in colon cancer
FASEB J. 2019
09. Tumour-vasculature development via endothelial-to-mesenchymal transition after radiotherapy controls CD44v6+ cancer cell and macrophage polarization
Nat Commun. 2018
10. CDKN2B deletion is essential for pancreatic cancer development instead of unmeaningful co-deletion due to juxtaposition to CDKN2A
Oncogene. 2018
11. KRASG12D and TP53R167H Cooperate to Induce Pancreatic Ductal Adenocarcinoma in Sus scrofa Pigs
Sci Rep. 2018
12. Antibody-mediated depletion of protein variant expression in living cells (Protein interference)
biorxiv , 2017
13. TGFá Signaling in the Pancreatic Tumor Microenvironment Promotes Fibrosis and Immune Evasion to Facilitate Tumorigenesis
Cancer Res. 2016
14. DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model
Gene Ther. 2014