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Arl1-GTP

Anti-Arl1-GTP Mouse Monoclonal Antibody
5/5

$495.00

Cat.#:  26924

   Size:   30 μl

In Stock

            Product Description          

Arl1-GTP

Cat. #: 26924
Size:  30 µL
Gene Symbol:  Arl1
Description:   Anti-Arl1-GTP Mouse Monoclonal Antibody
Background:   Arl1 (Arf-like 1) localizes to the Golgi complex, where it is believed to recruit effector proteins to the trans-Golgi network. Golgi-localized proteins golgin-97 and golgin-245 have been shown as Arl1 effectors. Additional Arl1 effectors include the GARP (Golgi-associated retrograde protein)/VFT (Vps53) vesicle-tethering complex and Arfaptin 2. In Drosophila zygotes, mutation of Arl1 is lethal.
Immunogen:   Recombinant Arl1 protein (active form)
Applications:   IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
Recommended Dilutions:   
IP: 1 µg for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative:  No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Arl1 antibody recognizes active Arl1-GTP from vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing

Immunoprecipitation/Western blot:  
Arl1 Activation Assay.

Purified GST-tagged Arl1 proteins (Cat. #10136) were immunoprecipitated with the anti-active Arl1 monoclonal antibody (Cat. # 26924) after treated with GDP (lane 1) or GTPγS (lane 2), and was blotted with anti- Arl1 monoclonal antibody (Cat. #26056). Arl1 protein input control is shown in the bottom panel.

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