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Anti-Gαi-GTP Mouse Monoclonal Antibody


Cat.#:  26901

   Size:   30 μl

In Stock

          Product Description          
Anti-GαiGTP Mouse Monoclonal Antibody
Gene Symbol:  Gnai
Description:   Anti-Gαi-GTP Mouse Monoclonal Antibody
Background:   Heterotrimeric G proteins are critical cellular signal transducers. Gαi represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gα i proteins are being explored.
Immunogen:   Recombinant full length protein of active Gα i1
Applications:   IP, IHC and IF (Not applicable for WB since SDS denatures Gαi GTPase)
Published Applications:  IP, IHC and IF – Click for Details
Recommended Dilutions:    IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG1
Purity:   Purified from ascites
Preservative:  No
Constituents:  PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-active Gα i antibody recognizes active Gα i1, Gα i2, and Gα i3 of vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing
Immunoprecipitation/Western blot: i activation assay. A. CHO cells were transfected with wild type Gα i1 (lanes 1 and 2) or constitutively active Gα i1-Q204L (lane 3). Cell lysates were treated with GDP(lane 1)or GTPγS (lane 3). Lysates were then incubated with an anti-active Gα i monoclonal antibody (Cat. No.26901) (top panel). The precipitated active Gα i was immunoblotted with an anti-Gα i monoclonal antibody (Cat. No. 26003). The bottom panel shows the Western blot with anti-Gα i monoclonal antibody (Cat.No. 26003) of the cell lysates. B. HEK293 cells stably expressing human m2 mAChR were treated with (lane 2) or without (lane 1)carbachol. Cell lysates were then incubated with an anti-active Gα i monoclonal antibody (Cat. No. 26901) (top panel).The precipitated active Gα i was immunoblotted with an anti-Gα i rabbit polyclonal antibody (Cat. No. 21006). The bottom panel shows the Western blot with anti-tubulin of the cell lysates.
Confocal microcopy images of RAW 264.7 cells expressing YFP-Gαi2QL or YFP-Gαi2 immunostained for GTP-Gαi delineated by phalloidin-Alexa Fluor 647 staining G-protein activation in HEK293 cells expressing CCR7 variants was visualized by confocal microscopy using an antibody specifically recognizing active, GTP-bound Gαi
01. Regulation of DNA damage response by trimeric G-proteins
iScience. 2023   PMID: 36756378
02. GPER mediates estrogen cardioprotection against epinephrine-induced stress
J Endocrinol. 2021  PMID: 33847279
03. Analgesic Efficacy of α2 Adrenergic Receptor Agonists Depends on the Chronic State of Neuropathic Pain: Role of Regulator of G Protein Signaling 4
Neuroscience. 2021  PMID: 33359660
04. Small Molecule Inhibitors Targeting Gαi2 Protein Attenuate Migration of Cancer Cells
Cancers. 2020  PMID: 32575572
05. Three-dimensional growth of breast cancer cells potentiates the anti-tumor effects of unacylated ghrelin and AZP-531
eLife. 2020  PMID: 32667883
06. Wnts regulate planar cell polarity via heterotrimeric G protein and PI3K signaling
J Cell Biol. 2020  PMID: 32805026
07. The COMMD3/8 complex determines GRK6 specificity for chemoattractant receptors
J Exp Med. 2019  PMID: 31088898
08. VLA-4 phosphorylation during tumor and immune cell migration relies on its coupling to VEGFR2 and CXCR4 by syndecan-1
J Cell Sci. 2019 2019  PMID: 31562188
09. Mechanoactivation of the angiotensin II type 1 receptor induces β‐arrestin‐biased signaling through Gαi coupling
J Cell Biochem. 2018  PMID: 29231251
10. Estrogen deficiency compromised the β 2 AR-Gs/Gi coupling: implications for arrhythmia and cardiac injury
Pflugers Arch. 2018  PMID: 29297096
11. Biased antagonism of CXCR4 avoids antagonist tolerance
Sci Signal. 2018   PMID: 30327409
12. Acupuncture Upregulates G Protein Coupled Activity in Samp8 Mice
Acupunct Med. 2017  PMID: 28450287
13. Gαi is required for carvedilol-induced β1 adrenergic receptor β-arrestin biased signaling
Nat Commun. 2017  PMID: 29167435
14. Sphingosine 1-phosphate (S1P) suppresses the collagen-induced activation of human platelets via S1P4 receptor
Thromb Res. 2017  PMID: 28609704
15. Wnt-5a/Frizzled9 Receptor Signaling through the Gαo-Gβγ Complex Regulates Dendritic Spine Formation
J Biol Chem. 2016  PMID: 27402827
16. Targeting brain tumor cAMP: the case for sex-specific therapeutics
Front. Pharmacol. 2015  PMID: 26283963
17. Rapid Actions of Xenoestrogens Disrupt Normal Estrogenic Signaling

Steroids. 2014  PMID: 24269739

18. The lipoprotein receptor LRP1 modulates sphingosine-1-phosphate signaling and is essential for vascular development
Development. 2014  PMID: 25377550
19. Neuronal NF1/RAS regulation of cyclic AMP requires atypical PKC activation
Hum Mol Genet. 2014  PMID: 25070947
20. Fatty Acid-binding Protein 5 (FABP5) Regulates Cognitive Function Both by Decreasing Anandamide Levels and by Activating the Nuclear Receptor Peroxisome Proliferator-activated Receptor ß/d (PPARß/d) in the Brain
J Biol Chem. 2014  PMID: 24644281
21. Structural basis for activation of trimeric Gi proteins by multiple growth factor receptors via GIV/Girdin
Mol Biol Cell. 2014  PMID: 25187647
22. Canonical and noncanonical g-protein signaling helps coordinate actin dynamics to promote macrophage phagocytosis of zymosan
Mol Cell Biol. 2014  PMID: 25225330
23. Identification of novel signalling roles and targets for Galpha and Gbetagamma downstream of the insulin-like growth factor 1 receptor in vascular smooth muscle cells
Biochem J. 2013  PMID: 23186281
24. Heterotrimeric Galpha(i) proteins are regulated by lipopolysaccharide and are anti-inflammatory in endotoxemia and polymicrobial sepsis
Biochim Biophys Acta. 2012  PMID: 21255617
25. CXCR4 activation defines a new subgroup of Sonic hedgehog-driven medulloblastoma
Cancer Res. 2012  PMID: 22052462
26. Regulation of Estradiol and Progesterone Production by CRH-R1 and -R2 Is through Divergent Signaling Pathways in Cultured Human Placental Trophoblasts
Endocrinology. 2012  PMID: 22865371
27. CRH activation of different signaling pathways results in differential calcium signaling in human pregnant myometrium before and during labor
J Clin Endocrinol Metab. 2012  PMID: 22869609
28. Estrogen- and xenoestrogen-induced ERK signaling in pituitary tumor cells involves estrogen receptor-à interactions with G protein-ài and caveolin I
Steroids. 2012  PMID: 22230296
29. WNT-5A stimulates the GDP/GTP exchange at pertussis toxin-sensitive heterotrimeric G proteins
Cell Signal. 2011  PMID: 21070854
30. Cannabinoids inhibit insulin receptor signaling in pancreatic β-cells
Diabetes.  2011  PMID: 21346174
31. PKA regulatory subunits mediate synergy among conserved G-protein-coupled receptor cascades
Nat Commun. 2011  PMID: 22186894
32. Na/H exchanger regulatory factors control parathyroid hormone receptor signaling by facilitating differential activation of G(alpha) protein subunits
J Biol Chem. 2010  PMID: 20562104
33. Beta-arrestin- but not G protein-mediated signaling by the “decoy” receptor CXCR7
Proc Natl Acad Sci U S A. 2010  PMID: 20018651