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Anti-SMO(A68V) Mouse Monoclonal Antibody


Cat.#:  26425

   Size:   100 μl

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          Product Description          
Cat. #:  26425
Size:  100 µL
Gene Symbol:  SMO; MRD21
Description:   Anti-SMO(A68V) Mouse Monoclonal Antibody
Background:   Smoothened is a protein that in humans is encoded by the SMO gene. Smoothened is a G protein-coupled receptor that is a component of the hedgehog signaling pathway and is conserved from flies to humans. It is the molecular target of the teratogen cyclopamine. SMO can function as an oncogene. Activating SMO mutations can lead to unregulated activation of the hedgehog pathway and cancer
Immunogen:   A synthetic peptide from the internal region of SMO which includes the mutation of A68V, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:    ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative: No
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes A68V mutant, but not wild type SMO of vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing
Western blot:   Western blot analysis of recombinant SMO(A68V) and wild type proteins. Purified His-tagged SMO(A68V) protein (lane 1) and corresponding wild type protein (lane 2) were blotted with Anti-SMO(A68V) monoclonal antibody (Cat. #26425).
Immunofluorescence:   Immunofluorescence of cells expressing SMO proteins with Anti-SMO(A68V) antibody. HEK293T cells were transfected with pCDNA3-GFP-SMO (WT) plasmid (left column) or pCDNA3-GFP-SMO(A68V) plasmid (right column), then fixed and stained with Anti-SMO(A68V) monoclonal antibody (Cat. #26425).
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