Email:   |  Call: 6109452007

cAMP EIA Kit – No Acetylation

Monoclonal Anti-cAMP Antibody Based Direct cAMP ELISA Kit


Cat.#:  80203

   Size:   96 well

In Stock

          Product Description          

Product Highlight

  • Based on the unique mouse monoclonal anti-cAMP antibody
  • The only assay kit on the market with monoclonal anti-cAMP antibody (all other kits are based on polyclonal anti-cAMP antibodies)
  • The monoclonal anti-cAMP antibody displays much higher selectivity over cGMP and ATP than polyclonal anti-cAMP antibody
  • Avoids the batch-to-batch variations associated with polyclonal antibody productions from animals, thus providing the reproducibility

Product Description

Adenosine 3′, 5′-cyclic monophosphate (cyclic AMP; cAMP) modulates various physiological functions such as cardiovascular biology, learning and memory, olfaction, immune response, asthma and kidney function (1,2). cAMP is produced from ATP by adenylyl cyclases and is degraded by phosphodiesterases. Stimulation of adenylyl cyclases or inhibition of phosphodiesterases can increase cellular cAMP concentrations. Blockers of adenylyl cyclase-activating receptors and inhibitors of the cAMP-specific phosphodiesterases are used for treating human diseases. For example, blocking agents for cAMP-increasing beta-adrenergic receptors (beta-blockers) are used for treating abnormal heart rhythms, high blood pressure (hypertension), myocardial infarction and heart failure. Inhibitors of cAMP specific phosphodiesterase types 2 and 4 are being tested for cognition enhancement.
To screen for inhibitors or stimulators of cellular cAMP levels, it is essential to have a sensitive, selective and reproducible method to measure the cAMP concentrations. This is especially true for the initial screenings given the possible weaker effects of larger pools of compounds.
Currently available other ELISA kits measuring cAMP levels are based on the non-affinity-purified polyclonal anti-cAMP antibody. Despite the claimed selectivity, these polyclonal anti-cAMP antibodies display certain cross-reactivity with ATP. Given that ATP is the substrate for the cAMP production, it is very desirable to have an antibody with high specificity towards cAMP over ATP.
NewEast Biosciences cAMP ELISA kit is based on the unique mouse monoclonal anti-cAMP antibody. This monoclonal anti-cAMP antibody displays >108 fold of selectivity over ATP, cGMP, and other nucleoside analogues. NewEast Biosciences cAMP ELISA kit provides significantly improved sensitivity and selectivity over other kits based on polyclonal anti-cAMP antibodies. Our monoclonal anti-cAMP antibody-based ELISA kit also avoids the batch-to-batch variations associated with polyclonal antibody productions from animals, thus providing the reproducibility in the long run.


Principle Outline

NewEast Biosciences cAMP ELISA Kit is a competitive immunoassay to measure the cAMP levels, either from cell extracts or from in vitro adenylyl cyclase assays. Briefly, multi-well plates are coated with goat-anti-mouse serum. cAMP in cell extracts or in in vitro adenylyl cyclase assays will competitively bind to the monoclonal anti-cAMP antibody in the presence of fixed amounts of cAMP-conjugated horse-radish peroxidase or alkaline phosphatase. Known amounts of cAMP are used as standards to generate the calculation curve. After a short incubation, the excess reagents are washed away and substrate is added. The multiwell plates are then read on a microplate reader at 450 nm or 405 nm. The intensity of the yellow color is inversely proportional to the concentration of cAMP in samples. The measured optical density is used to calculate the concentration of cAMP in samples based on the curve from the cAMP standards.
          Related Products          
01. A novel effector RipBT contributes to Ralstonia solanacearum virulence on potato
Mol Plant Pathol. 2023   PMID: 37154802
02. Comparative genomic analysis of Ralstonia solanacearum reveals candidate avirulence effectors in HA4-1 triggering wild potato immunity
Front Plant Sci. 2023   PMID: 36909411
03. Whole-Transcriptome Analysis Highlights Adenylyl Cyclase Toxins-Derived Modulation of NF-?B and ERK1/2 Pathways in Macrophages
Toxins (Basel). 2023   PMID: 36828453
04. Perfluorooctane sulfonate and perfluorooctanoic acid inhibit progesterone-responsive capacitation through cAMP/PKA signaling pathway and induce DNA damage in human sperm
Environmental Toxicology and Pharmacology 2023   PMID: 
05. Phosphodiesterase 10A (PDE10A) as a novel target to suppress β-catenin and RAS signaling in epithelial ovarian cancer
J Ovarian Res. 2022
06. Botrytis cinerea G Protein β Subunit Bcgb1 Controls Growth, Development and Virulence by Regulating cAMP Signaling and MAPK Signaling
J Fungi (Basel). 2021
07. Endogenous sulfur dioxide is a novel inhibitor of hypoxia-induced mast cell degranulation
J Adv Res. 2021
08. Glucocorticoids Elevate Pseudomonas aeruginosa Binding to Airway Epithelium by Upregulating Syndecan-1 Expression
Front Microbiol. 2021
09. Lipid biosynthesis mediated by the cyclic adenosine monophosphate (cAMP) signaling pathway in Chlorella pyrenoidosa under salt-induced osmotic stress
Renewable Energy, 2021
10. Maternal intake restriction programs the energy metabolism, clock circadian regulator and mTOR signals in the skeletal muscles of goat offspring probably via the protein kinase A-cAMP-responsive element-binding proteins pathway
Anim Nutr. 2021
11. Matrine Impairs Platelet Function and Thrombosis and Inhibits ROS Production
Front Pharmacol. 2021
12. MoWhi2 regulates appressorium formation and pathogenicity via the MoTor signalling pathway in Magnaporthe oryzae
Mol Plant Pathol. 2021
13. Serotonin receptor 4 in the hippocampus modulates mood and anxiety
Mol Psychiatry. 2021
14. Theacrine, a Potent Antidepressant Purine Alkaloid from a Special Chinese Tea, Promotes Adult Hippocampal Neurogenesis in Stressed Mice
J Agric Food Chem. 2021
15. Two forms of G protein-coupled estrogen receptor 1 in the ricefield eel: Expression and functional characterization in relation to ovarian follicle development
Gen Comp Endocrinol. 2021
16. Urocortins exhibit differential effects on PGE2 and PGF2α output via CRHR2 in human myometrium
Reproduction. 2021
17. Dual-activation of cAMP production through photo-stimulation or chemical stimulation
Methods Mol Biol. 2020
18. Homogeneous single-label cGMP detection platform for the functional study of nitric oxide-sensitive (soluble) guanylyl cyclases and cGMP-specific phosphodiesterases
Methods Mol Biol. 2020
19. Inhibition of DRG-TRPV1 upregulation in myocardial ischemia contributes to exogenous cardioprotection
J Mol Cell Cardiol. 2020
20. A systematic screen of conserved Ralstonia solanacearum effectors reveals the role of RipAB, a nuclear‐localized effector that suppresses immune responses in potato
Mol Plant Pathol. 2019
21. An improved method for isolation of mitochondria from cell lines that enables reconstitution of calcium-dependent processes
Anal Biochem. 2019
22. Expression patterns of C1ql4 and its cell-adhesion GPCR Bai3 in the murine testis and functional roles in steroidogenesis
FASEB J. 2019
23. Glucagon-like peptide-1 attenuates endothelial barrier injury in diabetes via cAMP/PKA mediated down-regulation of MLC phosphorylation
Biomed Pharmacother. 2019
24. Identification of expression and function of the glucagon-like peptide-1 receptor in colonic smooth muscle
Peptides. 2019
25. Luminescence-activated nucleotide cyclase regulates spatial and temporal cAMP synthesis
J Biol Chem. 2019
26. The cyclase-associated protein ChCAP is important for regulation of hyphal growth, appressorial development, penetration, pathogenicity, conidiation, intracellular cAMP level, and stress tolerance in Colletotrichum higginsianum
Plant Sci. 2019
27. A Type IIb, but Not Type IIa, GnRH Receptor Mediates GnRH-Induced Release of Growth Hormone in the Ricefield Eel
Front Endocrinol (Lausanne). 2018
28. Natriuretic peptide receptor 2 (NPR2) localized in bovine oocyte underlies a unique mechanism for C-type natriuretic peptide (CNP)-induced meiotic arrest
Theriogenology. 2018
29. DHA induces apoptosis of human malignant breast cancer tissues by the TLR-4/PPAR-α pathways
Oncol Lett. 2018
30. Improving heterologous expression of porcine follicle-stimulating hormone in Pichia pastoris by integrating molecular strategies and culture condition optimization
Appl Microbiol Biotechnol. 2018
31. N-octanoylated ghrelin peptide inhibits bovine oocyte meiotic resumption
Gen Comp Endocrinol. 2018
32. Environmental and genetic regulation of white-opaque switching in Candida tropicalis
Mol Microbiol. 2017
33. Influence of cell confluence on the cAMP signalling pathway in vascular smooth muscle cells
Cell Signal. 2017
34. Influence of dopamine D1 receptor Ala229Thr polymorphism on receptor signal transduction
中国临床药理学与治疗学. 2017
35. Integration of the tricarboxylic acid (TCA) cycle with cAMP signaling and Sfl2 pathways in the regulation of CO2 sensing and hyphal development in Candida albicans
PLoS Genet. 2017
36. Multifaceted Roles of the Ras Guanine-Nucleotide Exchange Factor ChRgf in Development, Pathogenesis, and Stress Responses of Colletotrichum higginsianum
Phytopathology. 2017
37. Zebrafish phosvitin-derived peptide Pt5 inhibits melanogenesis via cAMP pathway
Fish Physiol Biochem. 2017
38. A cardiac mitochondrial cAMP signaling pathway regulates calcium accumulation, permeability transition and cell death
Cell Death Dis. 2016
39. Characterization and expression of soluble guanylate cyclase in skins and melanocytes of sheep
Acta Histochem. 2016
40. Dietary flavonoid luteolin attenuates uropathogenic Escherichia. Coli invasion of the urinary bladder
Biofactors. 2016
41. Hypoxia-Inducible Factor-2α Limits Natural Killer T Cell Cytotoxicity in Renal Ischemia/Reperfusion Injury
J Am Soc Nephrol. 2016
42. MoRad6-mediated ubiquitination pathways are essential for development and pathogenicity in Magnaporthe oryzae
Environ Microbiol. 2016
43. Nuclear translocation of nuclear factor kappa B is regulated by G protein signaling pathway in arsenite-induced apoptosis in HBE cell line
Environ Toxicol. 2016
44. RNA polymerase III component Rpc9 regulates hematopoietic stem and progenitor cell maintenance in zebrafish
Development. 2016
45. The effects of IGF1 on the melanogenesis in alpaca melanocytes in vitro
In Vitro Cell Dev Biol Anim. 2016
46. Comparative Effects of Urocortins and Stresscopin on Cardiac Myocyte Contractilit
J Mol Cell Cardiol. 2015
47. Effect of dobutamine on lung aquaporin 5 in endotoxine shock-induced acute lung injury rabbit
J Thorac Dis. 2015
48. Identification of a novel microRNA important for melanogenesis in alpaca (Vicugna pacos)
J Anim Sci. 2015
49. Positive Effect of Carbon Sources on Natural Transformation in Escherichia coli: Role of Low-Level Cyclic AMP (cAMP)-cAMP Receptor Protein in the Derepression of rpoS
J Bacteriol. 2015
50. Alteration of vascular reactivity in heart failure: Role of phosphodiesterases type 3 and 4
Br J Pharmacol. 2014
51. cAMP–PKA inhibition of SK3 channel reduced both Ca2+ entry and cancer cell migration by regulation of SK3–Orai1 complex
Pflugers Arch. 2014
52. Comparative study of somatostatin-human serum albumin fusion proteins and natural somatostatin on receptor binding, internalization and activation
PLoS One. 2014
53. Immunomodulating effects of casein-derived peptide QEPVL and QEPV on lymphocytes in vitro and in vivo
Food Funct. 2014
54. Inactivation of the Carney complex gene 1 (PRKAR1A) alters spatiotemporal regulation of cAMP and cAMP-dependent protein kinase: a study using genetically encoded FRET-based reporters
Hum Mol Genet. 2014
55. Ischemia/Reperfusion-Induced CHOP Expression Promotes Apoptosis and Impairs Renal Function Recovery: The Role of Acidosis and GPR4
PLoS One. 2014
56. Sex is a major determinant of neuronal dysfunction in Neurofibromatosis Type 1
Ann Neurol. 2014
57. Three genes encoding citrate synthases in Saccharopolyspora erythraea are regulated by the global nutrient-sensing regulators GlnR, DasR, and CRP
Mol Microbiol. 2014
58. Opposing HDAC4 nuclear fluxes due to phosphorylation by β adrenergic activated PKA or by activity or Epac activated CaMKII in skeletal muscle fibres
J Physiol. 2013
59. Subendocardial Increase in Reactive Oxygen Species Production Affects Regional Contractile Function in Ischemic Heart Failure
Antioxid Redox Signal. 2013
60. Transgenic rescue of defective Cd36 enhances myocardial adenylyl cyclase signaling in spontaneously hypertensive rats
Pflugers Arch. 2013
61. Uncoupling of M1 muscarinic receptor/G-protein interaction by amyloid beta(1-42)
Neuropharmacology. 2013
62. Agonist-Dependent And -Independent Dopamine-1-Like Receptor Signalling Differently Regulates Downstream Effectors
Handb Exp Pharmacol. 2012
63. Antiarrhythmic effect of prolonged morphine exposure is accompanied by altered myocardial adenylyl cyclase signaling in rats
Pharmacol Rep. 2012
64. Heterogeneity in relaxation of different sized porcine coronary arteries to nitrovasodilators: role of PKG and MYPT1
Pflugers Arch. 2012
65. Natriuretic peptides block synaptic transmission by activating phosphodiesterase 2A and reducing presynaptic PKA activity
Proc Natl Acad Sci U S A. 2012
66. Structural basis of anthrax edema factor neutralization by a neutralizing antibody
Biochem Biophys Res Commun. 2012
67. Retigeric Acid B Attenuates the Virulence of Candida albicans via Inhibiting Adenylyl Cyclase Activity Targeted by Enhanced Farnesol Production
PLoS One. 2012
68. Molt-inhibiting hormone from Chinese mitten crab (Eriocheir sinensis): Cloning, tissue expression and effects of recombinant peptide on ecdysteroid secretion of YOs
Gen Comp Endocrinol. 2011