Pioneering GTPase and Oncogene Product Development since 2010

Email us: info@neweastbio.com

Call us: 6109452007

Rab5-GTP

Anti-Rab5-GTP Monoclonal Antibody
5/5

$495.00

Cat.#:  26911

   Size:   30 μl

In Stock

            Product Description          
Rab5-GTP
Cat. #:   26911
Size:  30 µL
Gene Symbol:  RAB5
Description:   Anti-Rab5-GTP Monoclonal Antibody
Background:   Rab5 is a member of Rab-family GTPases. Rab5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway.
Immunogen:   Recombinant full length protein of active Gao
Applications:   IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
Recommended Dilutions:    IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG1
Purity:   Purified from ascites
Preservative:  No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-active Rab5 antibody recognizes active Rab5 proteins of vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing
Immunoprecipitation/Western blot:   Rab5 Activation Assay.
Purified full-length Rab5A proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-Rab5-GTP monoclonal antibody (Cat. No. 26911). Immunoblot was with an anti-Rab5 polyclonal antibody (Cat. No. 21151).
          Related Products          
          Publications          
01. Celastrol Downmodulates Alpha-Synuclein-Specific T Cell Responses by Mediating Antigen Trafficking in Dendritic Cells
Front Immunol. 2022  PMID: 35309340
02. Preclinical and randomized clinical evaluation of the p38α kinase inhibitor neflamapimod for basal forebrain cholinergic degeneration
Nat Commun. 2022  PMID: 36130946
03. Assessing rab5 activation in health and disease
Methods Mol Biol.. 2021  PMID: 34453725
04. Role of Endocytosis Proteins in Gefitinib-Mediated EGFR Internalisation in Glioma Cells
Cells. 2021  PMID: 34831480
05. cGMP favors the interaction between APP and BACE1 by inhibiting Rab5 GTPase activity
Sci Rep.. 2020  PMID: 31992816
06. Discovery of a novel EGFR ligand DPBA that degrades EGFR and suppresses EGFR-positive NSCLC growth
Signal Transduct Target Ther. 2020  PMID: 33033232
07. Endosomal Dysfunction Induced by Directly Overactivating Rab5 Recapitulates Prodromal and Neurodegenerative Features of Alzheimer’s Disease
Cell Rep.. 2020  PMID: 33238112
08. High glucose-mediated PICALM and mTORC1 modulate processing of amyloid precursor protein via endosomal abnormalities
Br J Pharmacol. 2020  PMID: 32436237
09. NuMA1 promotes axon initial segment assembly through inhibition of endocytosis
J Cell Biol. 2020  PMID: 31727776
10. Protein diaphanous homolog 1 (Diaph1) promotes myofibroblastic activation of hepatic stellate cells by regulating Rab5a activity and TGFß receptor endocytosis
FASEB J. 2020  PMID: 32304339
11. ROR1 sustains multivesicular endosomes by interacting with HRS and STAM1
Research Square. 2020
12. TUFT1 promotes metastasis and chemoresistance in triple negative breast cancer through the TUFT1/Rab5/Rac1 pathway
Cancer Cell Int. 2019  PMID: 31572059
13. ZFYVE21 is a complement-induced Rab5 effector that activates non-canonical NF-?B via phosphoinosotide remodeling of endosomes
Nat Commun. 2019  PMID: 31113953
14. GAPVD1 and ANKFY1 Mutations Implicate RAB5 Regulation in Nephrotic Syndrome
J Am Soc Nephrol. 2018  PMID: 29959197
15. Ulk1 Governs Nerve Growth Factor/TrkA Signaling by Mediating Rab5 GTPase Activation in Porcine Hemagglutinating Encephalomyelitis Virus-Induced Neurodegenerative Disorders
J Virol. 2018  PMID: 29875237
16. Developmentally regulated GTP-binding protein 2 coordinates Rab5 activity and transferrin recycling
Mol Biol Cell. 2016  PMID: 26582392
17. Rin1 restores host phagocytic activity during invasion by Pseudomonas aeruginosa
J Med Microbiol. 2016  PMID: 26902911
18. AKT-independent Reelin signaling requires interactions of heterotrimeric Go and Src
Biochem Biophys Res Commun. 2015   PMID: 26441085
19. Central role for PICALM in amyloid–ß blood–brain barrier transcytosis and clearance
Nat Neurosci. 2015  PMID: 26005850
20. Intracellular Signaling and Trafficking in Cancer: Role of Rab5-GTPase in Migration and Invasion of Breast Cells
FIU. 2015
21. Rab5-mediated VE-cadherin internalization regulates the barrier function of the lung microvascular endothelium
Cell Mol Life Sci. 2015  PMID: 26112597
22. Rab5 Activity Regulates GLUT4 Sorting Into Insulin-Responsive and Non-Insulin-Responsive Endosomal Compartments: A Potential Mechanism for Development of Insulin Resistance
Endocrinology. 2014  PMID: 24932807