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Ras-GTP

Anti-Ras-GTP Monoclonal Antibody
5/5

$495.00

Cat.#:  26909

   Size:   30 μl

In Stock

            Product Description          
Ras-GTP
Cat. #:   26909
Size:  30 µL
Gene Symbol:  RAS
Description:   Anti-Ras-GTP Mouse Monoclonal Antibody
Background:   Small GTPases are a super-family of cellular signaling regulators. Ras belongs to the Ras sub-family of GTPases that regulate cell proliferation, cell motility, and gene transcription. GTP binding increases the activity of Ras, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).
Immunogen:   Recombinant full length protein of active H-Ras
Applications:   IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
Recommended Dilutions:    IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative:  No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-Ras-GTP monoclonal antibody recognizes active H-Ras, N-Ras, and K-Ras from vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing
Immunoprecipitation/Western blot:   IP/WB Analysis of Active Ras Protein.Purified recombinant Ras proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-Ras-GTP monoclonal antibody (Cat. # 26909). After SDS/PAGE, the membrane filter was probed with anti-Ras mouse monoclonal antibody (Cat. # 26014).
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01. microRNA-324-3p suppresses the aggressive ovarian cancer by targeting WNK2/RAS pathway
Bioengineered. 2022  PMID: 35549643
02. GPR110 promotes progression and metastasis of triple-negative breast cancer
Cell Death Discov. 2022  PMID: 35614051
03. Protection of dopaminergic neurons in hemiparkinsonian monkeys by flavouring ingredient glyceryl tribenzoate
NeuroImmune Pharm Ther. 2022  PMID: 0
04. KRAS Affects Adipogenic Differentiation by Regulating Autophagy and MAPK Activation in 3T3-L1 and C2C12 Cells
Int J Mol Sci. 2021  PMID: 34948427
05. A RAS inhibitor reduces allergic airway remodeling via regulating IL-33-derived type 2 innate lymphoid cells
Exp Lung Res. 2021  PMID: 34739349
06. A novel terpenoid class for prevention and treatment of KRAS-driven cancers: Comprehensive analysis using in situ, in vitro and in vivo model systems
Mol Carcinog. 2020  PMID: 32291806
07. Glycyrrhetinic acid binds to the conserved P-loop region and interferes with the interaction of RAS-effector proteins
Acta Pharm Sin B. 2019  PMID: 30976491
08. The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2
Mol Cell. 2019  PMID: 30639242
09. Glycyrrhetinic Acid Improves Insulin-Response Pathway by Regulating the Balance between the Ras/MAPK and PI3K/Akt Pathways
Nutrients. 2019  PMID: 30871060
10. Characterization of Novel Murine and Human PDAC Cell Models: Identifying the Role of Intestine Specific Homeobox Gene ISX in Hypoxia and Disease Progression
Transl Oncol. 2019  PMID: 31174057
11. ROS/KRAS/AMPK Signaling Contributes to Gemcitabine-Induced Stem-like Cell Properties in Pancreatic Cancer
Mol Ther Oncolytics. 2019  PMID: 31508487
12. KRAS regulation by small non-coding RNAs and SNARE proteins
Nat Commun. 2019  PMID: 31712554
13. Inhibition of Kirsten-Ras reduces fibrosis and protects against renal dysfunction in a mouse model of chronic folic acid nephropathy
Sci Rep. 2019  PMID: 31570767
14. Desmoplakin maintains gap junctions by inhibiting Ras/MAPK and lysosomal degradation of connexin-43
J Cell Biol. 2018  PMID: 29959233
15. GDF15 promotes the proliferation of cervical cancer cells by phosphorylating AKT1 and Erk1/2 through the receptor ErbB2
J Exp Clin Cancer Res. 2018  PMID: 29636108
16. miR-296-5p suppresses EMT of hepatocellular carcinoma via attenuating NRG1/ERBB2/ERBB3 signaling
J Exp Clin Cancer Res. 2018  PMID: 30486894
17. Lectin BS-I inhibits cell migration and invasion via AKT/GSK-3ß/ß-catenin pathway in hepatocellular carcinoma
J Cell Mol Med. 2018  PMID: 28922551
18. Biased signaling by thyroid-stimulating hormone receptor-specific antibodies determines thyrocyte survival in autoimmunity
Sci Signal. 2018  PMID: 29363585
19. Immunohistochemical evalulation of activated Ras and Rac1 as potential downstream effectors of aquaporin-5 in breast cancer in vivo
Biochem Biophys Res Commun. 2017  PMID: 28958942
20. Inhibition of lung cancer in diethylnitrosamine-induced mice by Rhizoma paridis saponins
Mol Carcinog. 2017  PMID: 27991692
21. Developmental stage-dependent regulation of spine formation by calcium-calmodulin-dependent protein kinase IIa and Rap1
Sci Rep. 2017  PMID: 29042611
22. p120RasGAP Protein Mediates Netrin-1 Protein-induced Cortical Axon Outgrowth and Guidance
J Biol Chem. 2016  PMID: 26710849
23. Sos1 Regulates Macrophage Podosome Assembly and Macrophage Invasive Capacity
J Immunol. 2015  PMID: 26447228
24. H-Ras regulation of TRAIL death receptor mediated apoptosis
Oncotarget. 2014  PMID: 25026275
25. Neurofibromin inactivation impairs osteocyte development in Nf1Prx1 and Nf1Col1 mouse models
Bone. 2014  PMID: 24947449
26. Depletion of the transcriptional coactivators megakaryoblastic leukaemia 1 and 2 abolishes hepatocellular carcinoma xenograft growth by inducing oncogene-induced senescence
EMBO Mol Med. 2013  PMID: 23853104
27. JNK inhibitors increase osteogenesis in Nf1-deficient cells
Bone. 2011  PMID: 21964323