NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$239.00
Vimentin Monoclonal Antibody
Cat. #: 26054 |
Gene Symbol: VIM |
Description: Anti-Vimentin Mouse Monoclonal Antibody |
Background: Vimentin is an intermediate filament protein found in various non-epithelial cells, especially in mesenchymal cells. It is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression. |
Immunogen: A synthetic peptide from the internal region of human vimentin protein. |
Applications: ELISA, WB, IHC, IP |
Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:100 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Anti-vimentin antibody recognizes vimentin of vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot: Western blot analysis of recombinant vimentin protein. Purified His-tagged vimentin protein (Cat. #10190) was blotted with Anti-vimentin monoclonal antibody (Cat. # 26054). lmmunohistochemistry: Immunohistochemical analysis of paraffin-embedded human renal cancer tissue with anti-vimentin monoclonal antibody (Cat. #26054). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:100 dilution) overnight at 4°C.A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody. |