Pioneering GTPase and Oncogene Product Development since 2010

Email us: info@neweastbio.com

Call us: 6109452007

Monoclonal Antibody Production Service

Mouse Monoclonal Antibody Service

Monoclonal antibodies (mAbs) have a much higher specificity and lower between-batch variability compared to pAbs.

Monoclonal antibodies are a class of antibodies that are produced by hybridoma cells by cell fusion technology and can bind to specific antigenic epitopes. Monoclonal antibodies have the characteristics of batch stability, high specificity and easy large-scale preparation, so they are widely used in biological research, in vitro diagnosis and drug development. According to the different needs of customers, we customize different solutions according to the type, quantity and purity of their antigens; through HAT screening and multiple subcloning screening to ensure 100% purity of cell lines; we use antibody affinity purification to ensure high antibody purity, Whether you use it downstream for diagnostic testing or drug research, its effectiveness is guaranteed.

StepDetailsTimeline
Antigen design and preparationRecombinant protein production and peptide synthesis and conjugation1-3 Weeks
First ImmunizationSubcutaneous multipoint immunization4 Week
First BoosterSubcutaneous multipoint immunization7 Weeks
Second BoosterSubcutaneous multipoint immunization9 Weeks
Blood Test50 µL of serum was collected from each mouse, and the titer was detected by ELISA10 Week
Third BoosterSubcutaneous multipoint immunization11 Week
Shock ImmunityIntraperitoneal or intravenous immunization12 Week
Cell FusionSelect the mice with the best immune effect for cell fusion13 Week
Cell ScreeningScreening of positive cell lines by ELISA and other experimental methods14 Week
Ascites preparation and purificationFor WB antibody
1、 5 BALB/c mice will be immunized, and the best mouse will be chosen for fusion.
2、 1-3 positive clones will be delivered. 3、 1 clone will be used to make ascites followed by purification.
For application-specific antibody (IHC, IF, IP, ChIP)
1、 10 mice will be immunized, and the best 2 mice will be chosen for fusion.
2、 1-3 positive clones will be delivered.
3、 3-5 clones will be used to make ascites followed by purification.
17 Week
Quality AppraisalAccording to the customer’s downstream application, carry out corresponding quality inspection21 Week
SubtypingSubtyping of all positive cell lines and purified antibodies22 Week

Remark

  • Host: BLAB/C mice;
  • Antigen requirements: concentration>1mg/ml, purity>85%, and the antigen solution does not contain organic solvents, tris, amino acids, etc.

Deliverables

  • 3-5 strains of hybridoma cells
  • > 5 mg purified antibody
  • 100 µL ascites stock solution
  • Project report, including a complete set of data on antigen production, hybridoma construction, screening, cloning and related testing

 

Polyclonal Antibody Production Service

Custom Polyclonal Antibody Production

We has more than ten years of experience in antibody preparation and is well versed in the key aspects of polyclonal antibody preparation. The host for the antibody can be  rabbits, mice, rats, guinea pigs, hamsters, goats, chickens or other species. The titer of polyclonal antibodies can reach more than 1:10000. Whether the antigen is recombinant proteins, peptides and virus particles, we can provide you with a complete set of solutions from target design, antigen preparation to  production, purification and quality testing, ensuring that the prepared antibodies can be effectively used in project research.

Highlights

  • Free Antigen Design – Based on experience, data, and characteristics of your desired target.

  • Wider Choice of Host Animals – Choose from SPF (specific-pathogen-free) host animals, including rabbit, mouse, rat, and guinea pig.
  • High Specificity – Antibodies are purified by Protein A and antigen affinity chromatography. When needed, we  can provide cross-purification to obtain a more specific antibody.
  • Guaranteed Result – 1 ng of protein antigen or 100 ng of peptide antigen is detected when the antibody is diluted at 1:6000.

Process Workflow

StepDetailsTimeline
Antigen design and preparationRecombinant protein production and peptide synthesis and conjugationWeek 1-3
Negative serum collectionCollect 3-5 ml control serum before immunizationWeek 2-3
First ImmunizationSubcutaneous multipoint immunizationWeek 2-3
First BoosterSubcutaneous multipointWeek 4-5
Second BoosterSubcutaneous multipointWeek 6-7
Blood Test50 µL of serum was collected from each mouse, and the titer was detected by ELISAWeeks 7-8
Third BoosterSubcutaneous multipoint immunizationWeeks 9-10
Final blood collection20 ml serum was collected from each rabbit, and the titer was detected by ELISA12 Week
Shock ImmunityEar vein immunityWeeks 11-12
Final blood collectionCollect 40-50 ml of blood from each rabbitWeek 12-13
Antiserum PurificationChoose protein A or antigen affinity purification according to customer requirementsWeek 14-15
Project DeliveryAntibody delivery and project production reportWeeks 15-16

Remark

  • Host: New Zealand White Rabbit
  • Antigen requirements: concentration>1mg/ml, purity>85%, and the antigen solution does not contain organic solvents, tris, amino acids, etc.

Deliverables

  • 200 μL pre-immune negative serum (50 μL for mice);
  • More than 1 mg of antigens or polypeptides prepared by Primetal;
  • 3-5 mg of antigen affinity-purified polyclonal antibody (concentration > 1 mg/mL);