$495.00
| Anti-Rab5GTP Monoclonal Antibody |
| Cat. #: 26911 |
| Size: 30 µL |
| Gene Symbol: RAB5 |
| Description: Anti-Rab5-GTP Monoclonal Antibody |
| Background: Rab5 is a member of Rab-family GTPases. Rab5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway. |
| Immunogen: Recombinant full length protein of active Gao |
| Applications: IP, IHC and IF (Not applicable for WB since SDS denatures Rab5 GTPase) |
| Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG1 |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-active Rab5 antibody recognizes active Rab5 proteins of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
| Immunoprecipitation/Western blot:
Rab5 Activation Assay. Purified full-length Rab5A proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-Rab5-GTP monoclonal antibody (Cat. No. 26911). Immunoblot was with an anti-Rab5 polyclonal antibody (Cat. No. 21151). |
