NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$239.00
Gαi Monoclonal Antibody
Cat. #: 26003 |
Gene Symbol: Gnai |
Description: Anti-Gαi Mouse Monoclonal Antibody |
Background: Heterotrimeric G proteins are critical cellular signal transducers. Gai represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gai proteins are being explored. |
Immunogen: Recombinant full length protein of Gαi1 |
Applications: ELISA, WB, IHC |
Recommended Dilutions: ELISA 1:1000-1:5000,WB 1:1000-1:2000,IHC 1:100-1:200 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Gαi antibody recognizes Gαi1, Gαi2, and Gαi3 of vertebrates |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot: Western blot analysis of Gi proteins. Gαi1 plasmid DNA was transiently transfected into HEK-293 cells. Whole-cell lysate was used for western blots. The monoclonal anti-Gαi antibody was pre-incubated without (lane 1) or with (lane 2) the antigen protein (Gαi1) to show the specificity. |