$495.00
EGFR(vIII)
Cat. #: 26176 |
Gene Symbol: EGFR/HER1/ERBB1 |
Description: Anti-EGFR(vIII) Mouse Monoclonal Antibody |
Background: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC gamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (NSCLC). |
Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of vIII, human origin. |
Applications: ELISA, WB, IF, IHC |
Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:1000-1:5000 IHC: 1:50-1:100 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Recognizes EGFR(vIII), but not wild type EGFR from vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot:
Western blot analysis of recombinant EGFR(vIII) and wild type proteins.
Purified His-tagged EGFR(vIII) protein (partial, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-EGFR(vIII) mouse monoclonal antibody (Cat. # 26176).
Immunofluorescence:
Immunofluorescence of cells expressing EGFR proteins with anti-EGFR(vIII) antibody. HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR(vIII) plasmid (right column), then fixed and stained with anti-EGFR(vIII) monoclonal antibody (Cat. #26176).