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Mouse CD40 Ligand Protein, hFc Tag

Recombinant mouse CD40L protein with N-terminal human Fc tag
5/5

$550.00

Cat.#:  12192

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     12192
Product Name:   Mouse CD40 Ligand Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   CD40-L;TRAP;CD154;Cd40lg
Target:    CD40 Ligand
UNIPROT ID:    P27548
Description:    Recombinant mouse CD40L protein with N-terminal human Fc tag
Background:     Cytokine that binds to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (By similarity). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching (PubMed:1374165).[UniProtKB/Swiss-Prot Function]
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 42.1 kDa after removal of the signal peptide. The apparent molecular mass of hFc-CD40L is approximately 40-55 kDa due to glycosylation.
Molecular Characterization:    hFc(Glu99-Ala330) Mouse CD40 Ligand(Gly115-Leu260)
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Mouse CD40 Ligand Protein, hFc Tag on SDS-PAGE under reducing condition.
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