| Cat.#: 28031 |
| Product Name: Anti-CTLA4 Reference Monoclonal Antibody |
| Synonyms: CTLA4;CD152 |
| Description: Anti-CTLA4 Reference Monoclonal Antibody |
| Background: Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals. |
| Applications: ELISA; Flow Cyt |
| Recommended Dilutions: ELISA 1:5000-10000; Flow Cyt 1:100 |
| Host Species: Homo sapiens |
| Isotype: IgG1 |
| Purification: Purified from cell culture supernatant by affinity chromatography |
| Species Reactivity: Human CTLA-4 |
| Constituents: Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). |
Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CTLA4, mFc-His tagged protein 11145 can bind Anti-CTLA4 Neutralizing antibody in a linear range of 0.13-16.0 ng/ml. | 
Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CTLA4, hFc tagged protein (11289) can bind Anti-CTLA4 Neutralizing antibody in a linear range of 0.64-80.0 ng/ml. In order to specifically detect 28031, mouse anti-human Fab-specific antibody was used as detection antibody. | 
Figure 3. HEK293 cell line transfected with irrelevant protein (B) and human CTLA4 (A) were surface stained with anti-CTLA4 neutralizing antibody 1µg/ml (ipilimumab) followed by Alexa 488-conjugated anti-human IgG secondary antibody. | |
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