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CD28 (DM64) Rabbit mAb

Anti-CD28 (DM64) Rabbit Monoclonal Antibody
5/5

$445.00

Cat.#:  28371

   Size:   100 μg

In Stock

          Product Description          
Cat.#:   28371
Product Name:   Anti-CD28(DM64) Rabbit Monoclonal Antibody
Synonyms:   CD28; Tp44
Description:    Anti-CD28 antibody(DM64) Rabbit Monoclonal Antibody
Background:    The protein encoded by this gene is essential for T-cell proliferation and survival; cytokine production; and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Applications:    ELISA; Flow Cyt
Recommended Dilutions:    ELISA 1:5000-10000; Flow Cyt 1:100
Host Species:    Rabbit
Isotype:    Rabbit IgG
Purification:    Purified from cell culture supernatant by affinity chromatography
Species Reactivity:    Human CD28
Constituents:    Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD28 protein, mFc-His tagged protein 11142 can bind Rabbit anti-CD28 monoclonal antibody (clone: DM64) in a linear range of 1-100 ng/ml.

Figure 2. Expi 293 cell line transfected with irrelevant protein  (A) and human CD28  (B) were surface stained with Rabbit anti-CD28 monoclonal antibody 1µg/ml ( clone: DM64) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Figure 3. Flow cytometry data of serially titrated Rabbit anti-CD28 monoclonal antibody ( clone: DM64) on Jurkat cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Figure 4. Affinity ranking of different Rabbit anti- CD28 mAb clones by titration of different concentration onto Jurkat cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
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