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CD27 (DM58) Rabbit mAb

Anti-CD27 (DM58) Rabbit Monoclonal Antibody
5/5

$445.00

Cat.#:  28359

   Size:   100 μg

In Stock

          Product Description          
Cat.#:   28359
Product Name:   Anti-CD27(DM58) Rabbit Monoclonal Antibody
Synonyms:   CD27; TNFRSF7; S152; T14; Tp55
Description:    Anti-CD27 antibody(DM58) Rabbit Monoclonal Antibody
Background:    The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70; and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8:JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA); a proapoptotic protein; can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
Applications:    ELISA; Flow Cyt
Recommended Dilutions:    ELISA 1:5000-10000; Flow Cyt 1:100
Host Species:    Rabbit
Isotype:    Rabbit IgG
Purification:    Purified from cell culture supernatant by affinity chromatography
Species Reactivity:    Human CD27
Constituents:    Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. Expi 293 cell line transfected with irrelevant protein  (A) and human CD27  (B) were surface stained with Rabbit anti-CD27 monoclonal antibody 1µg/ml ( clone: DM58) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Figure 2. Flow cytometry data of serially titrated Rabbit anti-CD27 monoclonal antibody ( clone: DM58) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Figure 3. Affinity ranking of different Rabbit anti-CD27 mAb clones by titration of different concentration onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
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