| Cat.#: 28347 |
| Product Name: Anti-CTLA-4(DM51) Rabbit Monoclonal Antibody |
| Synonyms: CTLA4; CD152 |
| Description: Anti-CTLA-4 antibody(DM51) Rabbit Monoclonal Antibody |
| Background: This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain; a transmembrane domain; and a cytoplasmic tail. Alternate transcriptional splice variants; encoding different isoforms; have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond; while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus; Graves disease; Hashimoto thyroiditis; celiac disease; systemic lupus erythematosus; thyroid-associated orbitopathy; and other autoimmune diseases. |
| Applications: ELISA; Flow Cyt |
| Recommended Dilutions: ELISA 1:5000-10000; Flow Cyt 1:100 |
| Host Species: Rabbit |
| Isotype: Rabbit IgG |
| Purification: Purified from cell culture supernatant by affinity chromatography |
| Species Reactivity: Human CTLA-4 |
| Constituents: Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). |
Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CTLA-4 protein, mFc-His tagged protein (11145) can bind Rabbit anti-CTLA-4 monoclonal antibody(clone: DM50) in a linear range of 1-100 ng/ml. |  
Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human CTLA-4 (B) were surface stained with Rabbit anti-CTLA-4 monoclonal antibody 1µg/ml (clone: DM50) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody. | 
Figure 3. Flow cytometry data of serially titrated Rabbit anti-CTLA-4 monoclonal antibody ( clone: DM51 ) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used. | 
Figure 4. Affinity ranking of different Rabbit anti-CTLA-4 mAb clones by titration of different concentration onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used. |
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