Email: info@neweastbio.com   |  Call: 6109452007

CTLA-4 (DM50) Rabbit mAb

Anti-CTLA-4 (DM50) Rabbit Monoclonal Antibody
5/5

$445.00

Cat.#:  28345

   Size:   100 μg

In Stock

          Product Description          
Cat.#:   28345
Product Name:   Anti-CTLA-4(DM50) Rabbit Monoclonal Antibody
Synonyms:   CTLA4; CD152
Description:    Anti-CTLA-4 antibody(DM50) Rabbit Monoclonal Antibody
Background:    This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain; a transmembrane domain; and a cytoplasmic tail. Alternate transcriptional splice variants; encoding different isoforms; have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond; while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus; Graves disease; Hashimoto thyroiditis; celiac disease; systemic lupus erythematosus; thyroid-associated orbitopathy; and other autoimmune diseases.
Applications:    ELISA; Flow Cyt
Recommended Dilutions:    ELISA 1:5000-10000; Flow Cyt 1:100
Host Species:    Rabbit
Isotype:    Rabbit IgG
Purification:    Purified from cell culture supernatant by affinity chromatography
Species Reactivity:    Human CTLA-4
Constituents:    Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CTLA4 protein, mFc-His tagged protein 11145 can bind Rabbit anti-CTLA4 monoclonal antibody ( clone: DM50) in a linear range of 1-100 ng/ml.

Figure 2. Expi 293 cell line transfected with irrelevant protein  (A) and human CTLA4  (B) were surface stained with Rabbit anti-CTLA4 monoclonal antibody 1µg/ml ( clone: DM50) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Figure 3. Flow cytometry data of serially titrated Rabbit anti-CTLA4 monoclonal antibody  (clone: DM50) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Figure 4. Affinity ranking of different Rabbit anti-CTLA4 mAb clones by titration of different concentration onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
          Publications