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ACE2 (DM47) Rabbit mAb

Anti-ACE2 (DM47) Rabbit Monoclonal Antibody
5/5

$445.00

Cat.#:  28340

   Size:   100 μg

In Stock

          Product Description          
Cat.#:   28340
Product Name:   Anti-ACE2(DM47) Rabbit Monoclonal Antibody
Synonyms:   ACE-2; ACEH; ACE2
Description:    Anti-ACE2 antibody(DM47) Rabbit Monoclonal Antibody
Background:    The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9; and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function; as well as fertility. In addition; the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.
Applications:    ELISA; Flow Cyt
Recommended Dilutions:    ELISA 1:5000-10000; Flow Cyt 1:100
Host Species:    Rabbit
Isotype:    Rabbit IgG
Purification:    Purified from cell culture supernatant by affinity chromatography
Species Reactivity:    Human ACE2
Constituents:    Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. Expi 293 cell line transfected with irrelevant protein (left) and human ACE2 (right) were surface stained with Rabbit anti-ACE2 monoclonal antibody 1µg/ml (clone: DM47) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Figure 2. Flow cytometry data of serially titrated Rabbit anti-ACE2 monoclonal antibody ( clone: DM47) on Expi 293 cell line transfected with human ACE2. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Figure 3. Affinity ranking of different Rabbit anti-ACE2 mAb clones by titration of different concentration onto Expi 293 cell line transfected with human ACE2. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
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