$495.00
| Cat. #: 26234 |
| Size: 100 µL |
| Gene Symbol: PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha |
| Description: Anti-PIK3CA(E542K) Mouse Monoclonal Antibody |
| Background: Phosphatidyl 3-kinases (PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival. It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer. |
| Immunogen: A synthetic peptide from the internal region of PIK3CA which includes the mutation of E542K, human origin. |
| Applications: ELISA, WB |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:2000 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes E542K mutant, but not wild type PIK3CA of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
| Western blot:
Western blot analysis of recombinant PIK3CA(E542K) and wild type proteins. Purified His-tagged PIK3CA(E542K) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-PIK3CA(E542K) monoclonal antibody (Cat. #26234). |
