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SPATA13 Rabbit pAb

Anti-SPATA13 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S210613

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S210613
Product Name:   Anti-SPATA13 Rabbit Polyclonal Antibody
Synonyms:   ASEF2; ARHGEF29; RP11-307N16.3
UNIPROT ID:   Q96N96 (Gene Accession – BC109291 )
Background:    Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Regulates cell migration and adhesion assembly and disassembly through a RAC1, PI3K, RHOA and AKT1-dependent mechanism. Increases both RAC1 and CDC42 activity, but decreases the amount of active RHOA. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression. Both the ABR and the SH3 domains contribute to maintaining the protein in an inhibited conformation by associating with the C-terminal tail. Binding of these domains to the C-terminal tail inhibits the activity of the protein by blocking a region that is required for its GEF activity.
Immunogen:    Fusion protein of human SPATA13
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 210613(SPATA13 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the fusion protein and then with 210613(Anti-SPATA13 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 210613(Anti-SPATA13 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D121280(Anti-SPATA13 Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Hela cells;
Primary antibody: 210613(SPATA13 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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