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GCLC Rabbit pAb

Anti-GCLC Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S211955

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S211955
Product Name:   Anti-GCLC Rabbit Polyclonal Antibody
Synonyms:   GCL; GCS; GLCL; GLCLC
UNIPROT ID:   P48506 (Gene Accession – BC022487 )
Background:    Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
Immunogen:    Fusion protein of human GCLC
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using 211955(GCLC Antibody) at a dilution of 1/35(Cytoplasm).

Immunohistochemistry analysis of paraffin-embedded Human colorectal cancer tissue using 211955(Anti-GCLC Antibody) at a dilution of 1/35.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: Raw264.7, A549, Hepg2 and Jurkat cell lysates;
Primary antibody: 211955(GCLC Antibody) at dilution 1/800;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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