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UNC45B Rabbit pAb

Anti-UNC45B Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S212074

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S212074
Product Name:   Anti-UNC45B Rabbit Polyclonal Antibody
Synonyms:   CMYA4; UNC45; CTRCT43; SMUNC45; UNC-45B
UNIPROT ID:   Q8IWX7 (Gene Accession – BC101063 )
Background:    This gene encodes a co-chaperone required for folding and accumulation of type II myosins. The protein consists of three tetratricopeptide repeat motifs at the N-terminus that form a complex with heat shock protein 90, a central region of unknown function that is conserved in all Unc-45 proteins, and a C-terminal Unc-45/Cro1/She4 domain. The protein is expressed at high levels in striated muscle, where its muscle myosin chaperone activity is dependent on heat shock protein 90 acting as a co-chaperone. A missense mutation in this gene has been associated with cataract development. Alternative splicing results in multiple transcript variants.
Immunogen:    Fusion protein of human UNC45B
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Developmental Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 212074(UNC45B Antibody) at a dilution of 1/65(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 212074(Anti-UNC45B Antibody) at dilution 1/65.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: Human heart tissue lysate;
Primary antibody: 212074(UNC45B Antibody) at dilution 1/600;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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