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ATP5PO Rabbit pAb

Anti-ATP5PO Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S212962

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S212962
Product Name:   Anti-ATP5PO Rabbit Polyclonal Antibody
Synonyms:   ATPO; OSCP; ATP5O; HMC08D05
UNIPROT ID:   P48047 (Gene Accession – BC021233 )
Background:    The protein encoded by this gene is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. The encoded protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance. [provided by RefSeq, Jul 2008]
Immunogen:    Fusion protein of human ATP5PO
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 212962(ATP5PO Antibody) at a dilution of 1/90(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 212962(Anti-ATP5PO Antibody) at dilution 1/90.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: SKOV3, Hela cell, Mouse liver tissue, Mouse heart tissue, Human placenta tissue, HepG2 cell, Human heart tissue lysates;
Primary antibody: 212962(ATP5PO Antibody) at dilution 1/1250;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 5 seconds
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