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SMARCA4 Rabbit pAb

Anti-SMARCA4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S213406

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S213406
Product Name:   Anti-SMARCA4 Rabbit Polyclonal Antibody
Synonyms:   BRG1, SNF2, SWI2, MRD16, RTPS2, BAF190, SNF2L4, SNF2LB, hSNF2b, BAF190A
UNIPROT ID:   P51532 (Gene Accession – NP_003063 )
Background:    The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Synthetic peptide of human SMARCA4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 200-1000;ELISA: 1000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cancer, Neuroscience, Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 213406(SMARCA4 Antibody) at a dilution of 1/50(Cytoplasm, Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 213406(Anti-SMARCA4 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 213406(Anti-SMARCA4 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with synthetic peptide and then with D160134(Anti-SMARCA4 Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 50 μg;
Lane 1-3: K562 cells, 293T cells, hepG2 cells;
Primary antibody: 213406(SMARCA4 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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