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GAD1 Rabbit pAb

Anti-GAD1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S213541

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S213541
Product Name:   Anti-GAD1 Rabbit Polyclonal Antibody
Synonyms:   GAD; SCP; CPSQ1
UNIPROT ID:   Q99259 (Gene Accession – NP_000808 )
Background:    This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.
Immunogen:    Synthetic peptide of human GAD1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Neuroscience, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gasrtic cancer tissue using 213541(GAD1 Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human gasrtic cancer tissue is first treated with the synthetic peptide and then with 213541(Anti-GAD1 Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 213541(Anti-GAD1 Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with synthetic peptide and then with D160382(Anti-GAD1 Antibody) at dilution 1/40.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: Human fetal brain tissue;
Primary antibody: 213541(GAD1 Antibody) at dilution 1/571;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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